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64 Cu and fluorescein labeled anti-miRNA peptide nucleic acids for the detection of miRNA expression in living cells.

التفاصيل البيبلوغرافية
العنوان: 64 Cu and fluorescein labeled anti-miRNA peptide nucleic acids for the detection of miRNA expression in living cells.
المؤلفون: Croci S; Clinical Immunology, Allergy, and Advanced Biotechnologies Unit, Diagnostic Imaging and Laboratory Medicine Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy., Manicardi A; Department of Chemistry, Live Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze, Parma, 43124, Italy.; Organic and Biomimetic Chemistry Research Group, Department of Organic Chemistry, Faculty of Science, Ghent University, Krijgslaan 281-S4, Gent, 9000, Belgium., Rubagotti S; Nuclear Medicine Unit, Oncology and Advanced Technologies Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy., Bonacini M; Clinical Immunology, Allergy, and Advanced Biotechnologies Unit, Diagnostic Imaging and Laboratory Medicine Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy., Iori M; Nuclear Medicine Unit, Oncology and Advanced Technologies Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy., Capponi PC; Nuclear Medicine Unit, Oncology and Advanced Technologies Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy., Cicoria G; Medical Physics Department, University Hospital 'S. Orsola-Malpighi', 40138, Bologna, Italy., Parmeggiani M; Clinical Immunology, Allergy, and Advanced Biotechnologies Unit, Diagnostic Imaging and Laboratory Medicine Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy., Salvarani C; Rheumatology Unit, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy.; Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy., Versari A; Nuclear Medicine Unit, Oncology and Advanced Technologies Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy., Corradini R; Department of Chemistry, Live Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze, Parma, 43124, Italy., Asti M; Nuclear Medicine Unit, Oncology and Advanced Technologies Department, Azienda Unità Sanitaria Locale-IRCCS di Reggio Emilia, 42123, Reggio Emilia, Italy. asti.mattia@ausl.re.it.
المصدر: Scientific reports [Sci Rep] 2019 Mar 04; Vol. 9 (1), pp. 3376. Date of Electronic Publication: 2019 Mar 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Antisense Elements (Genetics)/*chemistry , Copper Radioisotopes/*chemistry , Fluorescein/*chemistry , MicroRNAs/*analysis , Peptide Nucleic Acids/*chemistry, A549 Cells ; Antisense Elements (Genetics)/metabolism ; Antisense Elements (Genetics)/pharmacokinetics ; Cell Line, Tumor ; Diagnostic Imaging/methods ; Giant Cell Arteritis/diagnostic imaging ; Humans ; MicroRNAs/metabolism ; Positron-Emission Tomography ; RNA, Antisense/chemistry
مستخلص: MiRNAs are single stranded RNAs of 18-22 nucleotides. They are promising diagnostic and prognostic markers for several pathologies including tumors, neurodegenerative, cardiovascular and autoimmune diseases. In the present work the development and characterization of anti-miRNA radiolabeled probes based on peptide nucleic acids (PNAs) for potential non-invasive molecular imaging in vivo of giant cell arteritis are described. MiR-146a and miR-146b-5p were selected as targets because they have been found up-regulated in this disease. Anti-miR and scramble PNAs were synthesized and linked to carboxyfluorescein or DOTA. DOTA-anti-miR PNAs were then labelled with copper-64 ( 64 Cu) to function as non-invasive molecular imaging tools. The affinity of the probes for the targets was assessed in vitro by circular dichroism and melting temperature. Differential uptake of fluorescein and 64 Cu labeled anti-miRNA probes was tested on BCPAP and A549 cell lines, expressing different levels of miR-146a and -146b-5p. The experiments showed that the anti-miR-146a PNAs were more effective than the anti-miR-146b-5p PNAs. Anti-miR-146a PNAs could bind both miR-146a and miR-146b-5p. The uptake of fluorescein and 64 Cu labeled anti-miR-146a PNAs was higher than that of the negative control scramble PNAs in miRNA expressing cells in vitro. 64 Cu-anti-miR-146a PNAs might be further investigated for non-invasive PET imaging of miR-146 overexpressing diseases.
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المشرفين على المادة: 0 (Antisense Elements (Genetics))
0 (Copper Radioisotopes)
0 (MIRN146 microRNA, human)
0 (MicroRNAs)
0 (Peptide Nucleic Acids)
0 (RNA, Antisense)
TPY09G7XIR (Fluorescein)
تواريخ الأحداث: Date Created: 20190306 Date Completed: 20200922 Latest Revision: 20240714
رمز التحديث: 20240714
مُعرف محوري في PubMed: PMC6399270
DOI: 10.1038/s41598-018-35800-x
PMID: 30833583
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-018-35800-x