دورية أكاديمية
أعرض في EDS

Hypotonicity differentially affects inflammatory marker production by nucleus pulposus tissue in simulated disc degeneration versus herniation.

التفاصيل البيبلوغرافية
العنوان: Hypotonicity differentially affects inflammatory marker production by nucleus pulposus tissue in simulated disc degeneration versus herniation.
المؤلفون: Mouser VHM; Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., Arkesteijn ITM; Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., van Dijk BGM; Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands., Wuertz-Kozak K; Institute for Biomechanics, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland.; Department of Health Sciences, University of Potsdam, Potsdam, Germany.; Schön Clinic Munich Harlaching (Academic Teaching Hospital and Spine Research Institute of the Paracelsus Medical University in Salzburg), Munich, Germany., Ito K; Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.; Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands.
المصدر: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2019 May; Vol. 37 (5), pp. 1110-1116. Date of Electronic Publication: 2019 Apr 01.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 8404726 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-527X (Electronic) Linking ISSN: 07360266 NLM ISO Abbreviation: J Orthop Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2006- : Hoboken, NJ : Wiley
Original Publication: New York, N.Y. : Raven Press, [c1983-
مواضيع طبية MeSH: Osmotic Pressure*, Biomarkers/*metabolism , Intervertebral Disc Degeneration/*metabolism , Intervertebral Disc Displacement/*metabolism , Nucleus Pulposus/*metabolism, Animals ; Cattle ; DNA/metabolism ; Extracellular Matrix Proteins/metabolism ; Inflammation/metabolism
مستخلص: Inflammatory cytokines play an important role in intervertebral disc degeneration. Although largely produced by immune cells, nucleus pulposus (NP) cells can also secrete them under various conditions, for example, under free swelling. Thus, tissue hypotonicity may be an inflammatory trigger for NP cells. The aim of this study was to investigate whether decreased tonicity under restricted swelling conditions (as occurring in early disc degeneration) could initiate an inflammatory cascade that mediates further degeneration. Healthy bovine NP tissue was balanced against different PEG concentrations (0-30%) to obtain various tissue tonicities. Samples were then placed in an artificial annulus (fixed volume) and were cultured for 3, 7, or 21 days, with free swelling NP as control. Tissue content (water, glycosaminoglycan, collagen) was analyzed, and both the tissue and medium were screened for tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), prostaglandin-E 2 (PGE 2 ), and nitric oxide (NO). A range of tonicities (isotonic to hypotonic) was present at day 3 in the PEG-treated samples. However, during culture, the tonicity range narrowed as GAGs leached from the tissue. TNF-α and IL-1β were below detection limits in all conditions, while mid- and downstream inflammatory cytokines were detected. This may suggest that the extracellular environment directly affects NP cells instead of inducing a classical inflammatory cascade. Furthermore, IL-8 increased in swelling restricted samples, while IL-6 and PGE 2 were elevated in free swelling controls. These findings may suggest the involvement of different mechanisms in disc degeneration with intact AF compared to herniation, and encourage further investigation. © 2019 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res.
(© 2019 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society.)
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فهرسة مساهمة: Keywords: (hypo)tonicity; inflammation; intervertebral disc degeneration; nucleus pulposus
المشرفين على المادة: 0 (Biomarkers)
0 (Extracellular Matrix Proteins)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20190306 Date Completed: 20191220 Latest Revision: 20200309
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6593810
DOI: 10.1002/jor.24268
PMID: 30835843
قاعدة البيانات: MEDLINE
الوصف
تدمد:1554-527X
DOI:10.1002/jor.24268