دورية أكاديمية
Interpreting a major component from a mixed DNA profile with an unknown number of minor contributors.
| العنوان: | Interpreting a major component from a mixed DNA profile with an unknown number of minor contributors. |
|---|---|
| المؤلفون: | Bille T; Bureau of Alcohol, Tobacco, Firearms, and Explosives Laboratory, National Laboratory Center, 6000 Ammendale Road, Beltsville, MD, 20705, United States. Electronic address: todd.bille@atf.gov., Weitz S; Bureau of Alcohol, Tobacco, Firearms, and Explosives Laboratory, National Laboratory Center, 6000 Ammendale Road, Beltsville, MD, 20705, United States., Buckleton JS; Institute of Environmental Science and Research Limited, Private Bag 92021, Auckland, 1142, New Zealand; University of Auckland, Department of Statistics, Private Bag 92019, Auckland, New Zealand., Bright JA; Institute of Environmental Science and Research Limited, Private Bag 92021, Auckland, 1142, New Zealand. |
| المصدر: | Forensic science international. Genetics [Forensic Sci Int Genet] 2019 May; Vol. 40, pp. 150-159. Date of Electronic Publication: 2019 Feb 22. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101317016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0326 (Electronic) Linking ISSN: 18724973 NLM ISO Abbreviation: Forensic Sci Int Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | DNA/*analysis , DNA Fingerprinting/*methods, Gene Frequency ; Humans ; Likelihood Functions ; Microsatellite Repeats ; Polymerase Chain Reaction |
| مستخلص: | Modern interpretation strategies typically require an assignment of the number of contributors (N) to a DNA profile. This can prove to be a difficult task, particularly when dealing with higher order mixtures or mixtures where one or more contributors have donated low amounts of DNA. Differences in the assigned N at interpretation can lead to differences in the likelihood ration (LR). If the number of contributors cannot reasonably be assigned, then an interpretation of the profile may not be able to be progressed. In this study, we investigate mixed DNA profiles of varying complexity and interpret them altering the assigned N. We assign LRs for true- and non- contributors and compare the results given different assignments of N over a range of mixture proportions. When a component of a mixture had a proportion of at least 10%, a ratio of at least 1.5:1 to the next highest component, and a DNA amount (as determined by STRmix™) of at least 50 rfu, the LR of the component for a true contributor was not significantly affected by varying N and was therefore suitable for interpretation and the assignment of an LR. LRs produced for minor contributors were found to vary significantly as the assigned N was changed. These heuristics may be used to identify profiles suitable for interpretation. (Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Forensic DNA; Mixture interpretation; Probabilistic genotyping; STRmix™ |
| المشرفين على المادة: | 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20190308 Date Completed: 20190425 Latest Revision: 20190425 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.fsigen.2019.02.017 |
| PMID: | 30844683 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1878-0326 |
|---|---|
| DOI: | 10.1016/j.fsigen.2019.02.017 |