دورية أكاديمية
أعرض في EDS

Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone.

التفاصيل البيبلوغرافية
العنوان: Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone.
المؤلفون: Padavannil A; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, United States., Sarkar P; Department of Biological Sciences, University of Texas at Dallas, Richardson, United States., Kim SJ; Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea., Cagatay T; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, United States., Jiou J; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, United States., Brautigam CA; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, United States., Tomchick DR; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, United States., Sali A; Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, United States.; Department of Pharmaceutical Chemistry, California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, United states., D'Arcy S; Department of Chemistry and Biochemistry, University of Texas at Dallas, Richardson, United States., Chook YM; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, United States.
المصدر: ELife [Elife] 2019 Mar 11; Vol. 8. Date of Electronic Publication: 2019 Mar 11.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: Histones/*chemistry , Histones/*metabolism , Karyopherins/*chemistry , Karyopherins/*metabolism, Active Transport, Cell Nucleus ; Animals ; Crystallography, X-Ray ; DNA Mutational Analysis ; Humans ; Karyopherins/genetics ; Molecular Chaperones/chemistry ; Molecular Chaperones/genetics ; Molecular Chaperones/metabolism ; Mutant Proteins/chemistry ; Mutant Proteins/genetics ; Mutant Proteins/metabolism ; Protein Binding ; Protein Conformation ; Xenopus
مستخلص: We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9•H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9•H2A-H2B but assembles into a RanGTP•Importin-9•H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome.
Competing Interests: AP, PS, SK, TC, JJ, CB, DT, AS, SD, YC No competing interests declared
(© 2019, Padavannil et al.)
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معلومات مُعتمدة: R01GM112108 United States GM NIGMS NIH HHS; S10 OD021512 United States OD NIH HHS; R01 GM083960 United States GM NIGMS NIH HHS; P41GM109824 United States GM NIGMS NIH HHS; S10 OD025018 United States OD NIH HHS; R01 GM112108 United States GM NIGMS NIH HHS; R01GM083960 United States GM NIGMS NIH HHS; R01GM069909 United States GM NIGMS NIH HHS; P41 GM103393 United States GM NIGMS NIH HHS; U01GM98256-01 United States GM NIGMS NIH HHS; P41 RR001209 United States RR NCRR NIH HHS; P41 GM109824 United States GM NIGMS NIH HHS; S10 OD021596 United States OD NIH HHS; R01 GM069909 United States GM NIGMS NIH HHS; U01 GM098256 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: E. coli; H2A-H2B, Ran; Importin-9; histone; histone chaperone; karyopherin; molecular biophysics; nucleosome; structural biology
المشرفين على المادة: 0 (Histones)
0 (IPO9 protein, human)
0 (Karyopherins)
0 (Molecular Chaperones)
0 (Mutant Proteins)
تواريخ الأحداث: Date Created: 20190312 Date Completed: 20200319 Latest Revision: 20240216
رمز التحديث: 20240216
مُعرف محوري في PubMed: PMC6453568
DOI: 10.7554/eLife.43630
PMID: 30855230
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.43630