دورية أكاديمية
Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract.
العنوان: | Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract. |
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المؤلفون: | Etame RME; Institute of Medical Research and Medicinal Plant Studies, (IMPM), PO, Box 13033, Yaoundé, Cameroon., Mouokeu RS; Institute of Fisheries and Aquatic Sciences, University of Douala, PO, Box 7236, Douala, Cameroon., Poundeu FSM; Faculty of Sciences, University of Douala, PO, Box 24157, Douala, Cameroon., Voukeng IK; Faculty of Sciences, University of Dschang, PO, Box 67, Dschang, Cameroon., Cidjeu CLP; Faculty of Sciences, University of Douala, PO, Box 24157, Douala, Cameroon., Tiabou AT; Institute of Medical Research and Medicinal Plant Studies, (IMPM), PO, Box 13033, Yaoundé, Cameroon., Yaya AJG; Institute of Medical Research and Medicinal Plant Studies, (IMPM), PO, Box 13033, Yaoundé, Cameroon., Ngane RAN; Faculty of Sciences, University of Douala, PO, Box 24157, Douala, Cameroon. angono@yahoo.com., Kuiate JR; Faculty of Sciences, University of Dschang, PO, Box 67, Dschang, Cameroon., Etoa FX; Faculty of Sciences, University of Yaoundé I, PO, Box 812, Yaoundé, Cameroon. |
المصدر: | BMC complementary and alternative medicine [BMC Complement Altern Med] 2019 Mar 11; Vol. 19 (1), pp. 56. Date of Electronic Publication: 2019 Mar 11. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101088661 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6882 (Electronic) Linking ISSN: 14726882 NLM ISO Abbreviation: BMC Complement Altern Med Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central, [2001-2019] |
مواضيع طبية MeSH: | Annonaceae/*chemistry , Anti-Bacterial Agents/*chemistry , Anti-Bacterial Agents/*pharmacology , Plant Extracts/*chemistry , Plant Extracts/*pharmacology, 1-Butanol/chemistry ; Bacteria/drug effects ; Magnetic Resonance Spectroscopy ; Microbial Sensitivity Tests ; Plant Bark/chemistry |
مستخلص: | Background: Enantia chlorantha is a plant belonging to Annonaceae Family. The Barks and leaves are used traditionally to treat infectious diseases. Earlier studies highlighted the antibacterial activity of stem barks methanol extract. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of its n-butanol fraction. Methods: The extract of E. chlorantha stem barks was obtained by maceration in methanol and then subjected to a liquid/liquid partition by successive depletion with solvents of increasing polarity. The n-butanol fraction was fractionated by adsorption chromatography on silica gel. A product was isolated from the dichloromethane/methanol (2%) fraction and the structure was determined on the basis of spectroscopic data; Proton Nuclear Magnetic Resonance ( 1 H NMR), Carbon-13 Nuclear Magnetic Resonance ( 13 C NMR), Heteronuclear Multiple Bond Correlation (HMBC), H-correlation spectroscopy (H-COSY), attached proton test (APT), heteronuclear multiple quantum coherence (HSQC). The antibacterial activity was evaluated by broth microdilution method against six reference strains and eight clinical bacterial strains. Results: The n-butanol fraction was found to be active with MIC values ranging from 32 to 256 μg/mL. The F Conclusion: The F |
References: | Mol Aspects Med. 2006 Feb;27(1):1-93. (PMID: 16105678) Nat Prod Rep. 2009 Jun;26(6):746-57. (PMID: 19471683) Planta Med. 2010 Oct;76(14):1479-91. (PMID: 20533165) Appl Microbiol Biotechnol. 2011 Nov;92(3):479-97. (PMID: 21904817) Ann Biol Clin (Paris). 2012 Nov-Dec;70(6):635-42. (PMID: 23207806) BMC Complement Altern Med. 2013 Jul 10;13:164. (PMID: 23837916) Acta Pol Pharm. 2015 Jan-Feb;72(1):147-52. (PMID: 25850210) BMC Complement Altern Med. 2015 Feb 15;15:25. (PMID: 25880310) BMC Complement Altern Med. 2017 Jan 7;17(1):28. (PMID: 28061888) Evid Based Complement Alternat Med. 2018 Apr 29;2018:4831593. (PMID: 29853954) |
فهرسة مساهمة: | Keywords: Antibacterial activity; Enantia chlorantha; Palmitin |
المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Plant Extracts) 8PJ61P6TS3 (1-Butanol) |
تواريخ الأحداث: | Date Created: 20190315 Date Completed: 20190410 Latest Revision: 20200225 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC6416886 |
DOI: | 10.1186/s12906-019-2459-y |
PMID: | 30866907 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1472-6882 |
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DOI: | 10.1186/s12906-019-2459-y |