دورية أكاديمية
أعرض في EDS

Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract.

التفاصيل البيبلوغرافية
العنوان: Effect of fractioning on antibacterial activity of n-butanol fraction from Enantia chlorantha stem bark methanol extract.
المؤلفون: Etame RME; Institute of Medical Research and Medicinal Plant Studies, (IMPM), PO, Box 13033, Yaoundé, Cameroon., Mouokeu RS; Institute of Fisheries and Aquatic Sciences, University of Douala, PO, Box 7236, Douala, Cameroon., Poundeu FSM; Faculty of Sciences, University of Douala, PO, Box 24157, Douala, Cameroon., Voukeng IK; Faculty of Sciences, University of Dschang, PO, Box 67, Dschang, Cameroon., Cidjeu CLP; Faculty of Sciences, University of Douala, PO, Box 24157, Douala, Cameroon., Tiabou AT; Institute of Medical Research and Medicinal Plant Studies, (IMPM), PO, Box 13033, Yaoundé, Cameroon., Yaya AJG; Institute of Medical Research and Medicinal Plant Studies, (IMPM), PO, Box 13033, Yaoundé, Cameroon., Ngane RAN; Faculty of Sciences, University of Douala, PO, Box 24157, Douala, Cameroon. angono@yahoo.com., Kuiate JR; Faculty of Sciences, University of Dschang, PO, Box 67, Dschang, Cameroon., Etoa FX; Faculty of Sciences, University of Yaoundé I, PO, Box 812, Yaoundé, Cameroon.
المصدر: BMC complementary and alternative medicine [BMC Complement Altern Med] 2019 Mar 11; Vol. 19 (1), pp. 56. Date of Electronic Publication: 2019 Mar 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101088661 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6882 (Electronic) Linking ISSN: 14726882 NLM ISO Abbreviation: BMC Complement Altern Med Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-2019]
مواضيع طبية MeSH: Annonaceae/*chemistry , Anti-Bacterial Agents/*chemistry , Anti-Bacterial Agents/*pharmacology , Plant Extracts/*chemistry , Plant Extracts/*pharmacology, 1-Butanol/chemistry ; Bacteria/drug effects ; Magnetic Resonance Spectroscopy ; Microbial Sensitivity Tests ; Plant Bark/chemistry
مستخلص: Background: Enantia chlorantha is a plant belonging to Annonaceae Family. The Barks and leaves are used traditionally to treat infectious diseases. Earlier studies highlighted the antibacterial activity of stem barks methanol extract. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of its n-butanol fraction.
Methods: The extract of E. chlorantha stem barks was obtained by maceration in methanol and then subjected to a liquid/liquid partition by successive depletion with solvents of increasing polarity. The n-butanol fraction was fractionated by adsorption chromatography on silica gel. A product was isolated from the dichloromethane/methanol (2%) fraction and the structure was determined on the basis of spectroscopic data; Proton Nuclear Magnetic Resonance ( 1 H NMR), Carbon-13 Nuclear Magnetic Resonance ( 13 C NMR), Heteronuclear Multiple Bond Correlation (HMBC), H-correlation spectroscopy (H-COSY), attached proton test (APT), heteronuclear multiple quantum coherence (HSQC). The antibacterial activity was evaluated by broth microdilution method against six reference strains and eight clinical bacterial strains.
Results: The n-butanol fraction was found to be active with MIC values ranging from 32 to 256 μg/mL. The F A sub-fraction was more efficient among the eight sub-fractions, the n-butanol fraction and comparable to Chloramphenicol used as reference antibiotic. The product obtained was elucidated as palmitin. The antibacterial activity of the latter was comparable to that of Chloramphenicol on one reference strain and 4 of the 6 clinical strains.
Conclusion: The F A sub-fraction had better antibacterial activity than the n-butanol fraction and other sub-fractions, and possibly palmitin was the active substance responsible for the antibacterial activity of E. chlorantha.
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فهرسة مساهمة: Keywords: Antibacterial activity; Enantia chlorantha; Palmitin
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Plant Extracts)
8PJ61P6TS3 (1-Butanol)
تواريخ الأحداث: Date Created: 20190315 Date Completed: 20190410 Latest Revision: 20200225
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6416886
DOI: 10.1186/s12906-019-2459-y
PMID: 30866907
قاعدة البيانات: MEDLINE
الوصف
تدمد:1472-6882
DOI:10.1186/s12906-019-2459-y