دورية أكاديمية
Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae.
| العنوان: | Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae. |
|---|---|
| المؤلفون: | Ferrer MJ; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, 'Dr. Héctor Torres' (INGEBI-CONICET), Buenos Aires, Argentina., Wehrendt DP; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, 'Dr. Héctor Torres' (INGEBI-CONICET), Buenos Aires, Argentina., Bonilla M; Group Redox Biology of Trypanosomes, Institut Pasteur de Montevideo, Montevideo, Uruguay., Comini MA; Group Redox Biology of Trypanosomes, Institut Pasteur de Montevideo, Montevideo, Uruguay., Tellez-Iñón MT; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, 'Dr. Héctor Torres' (INGEBI-CONICET), Buenos Aires, Argentina., Potenza M; Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, 'Dr. Héctor Torres' (INGEBI-CONICET), Buenos Aires, Argentina. potenza@dna.uba.ar. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1955, pp. 105-118. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Antigens, Protozoan/*genetics , Cloning, Molecular/*methods , Leishmania/*genetics , Trypanosoma cruzi/*genetics, Chagas Disease/parasitology ; Genetic Vectors/genetics ; Humans ; Plasmids/genetics ; Recombinant Proteins/genetics ; Transfection/methods |
| مستخلص: | Trypanosomatids are unicellular organisms that colonize a wide diversity of environments and hosts. For instance, Trypanosoma cruzi is a human pathogen responsible for Chagas diseases, while Leishmania tarentolae infects amphibians and became a biotechnological tool suitable for recombinant protein expression. T. cruzi antigens are needed for the development of improved epitope-based methods for diagnosis and treatment of Chagas disease. Molecular cloning for the production of recombinant proteins offers the possibility to obtain T. cruzi antigens at high yield and purity. L. tarentolae appears as the ideal expression host to obtain recombinant T. cruzi antigens with a structure and posttranslational modifications typical of trypanosomatids. In this chapter, we present a protocol for the analytical to mid-scale production of recombinant T. cruzi antigens, using L. tarentolae as expression host (LEXSY ® inducible system). |
| فهرسة مساهمة: | Keywords: Eukaryotic expression system; Leishmania tarentolae; Recombinant antigen; Trypanosoma cruzi |
| المشرفين على المادة: | 0 (Antigens, Protozoan) 0 (Recombinant Proteins) |
| تواريخ الأحداث: | Date Created: 20190315 Date Completed: 20190724 Latest Revision: 20190724 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-4939-9148-8_8 |
| PMID: | 30868522 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
|---|---|
| DOI: | 10.1007/978-1-4939-9148-8_8 |