دورية أكاديمية
Sodium butyrate inhibits planktonic cells and biofilms of Trichosporon spp.
| العنوان: | Sodium butyrate inhibits planktonic cells and biofilms of Trichosporon spp. |
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| المؤلفون: | Cordeiro RA; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil; Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza-CE, Brazil., Aguiar ALR; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil., Pereira VS; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil., Pereira LMG; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil., Portela FVM; Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza-CE, Brazil., Brilhante RSN; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil; Postgraduate Program in Medical Sciences, Federal University of Ceará, Fortaleza-CE, Brazil., Camargo ZP; Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo-SP, Brazil., Sidrim JJC; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil., Castelo-Branco DSCM; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil. Electronic address: deb.castelobranco@ufc.br., Rocha MFG; Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará, Fortaleza-CE, Brazil; School of Veterinary, Postgraduate Program in Veterinary Science, State University of Ceará, Fortaleza-CE, Brazil. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2019 May; Vol. 130, pp. 219-225. Date of Electronic Publication: 2019 Mar 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Antifungal Agents/*pharmacology , Biofilms/*drug effects , Butyric Acid/*pharmacology , Trichosporon/*drug effects, Cell Adhesion/drug effects ; Microbial Sensitivity Tests ; Microscopy ; Microscopy, Confocal ; Microscopy, Electron, Scanning ; Trichosporon/cytology ; Trichosporon/growth & development |
| مستخلص: | Trichosporon spp. have been increasingly recognized as an important pathogen of invasive and disseminated infections in immunocompromised patients. These species are prone to form biofilms in medical devices such as catheters and prosthesis, which are associated with antifungal resistance and therapeutic failure. Therefore, new antifungals with a broader anti-biofilm activity need to be discovered. In the present study we evaluate the inhibitory potential of sodium butyrate (NaBut) - a histone deacetylase inhibitor that can alter chromatin conformation - against planktonic and sessile cells of T. asahii and T. inkin. Minimum inhibitory concentration (MIC) of NaBut against planktonic cells was evaluated by microdilution and morphological changes were analyzed by optical microscopy on malt agar supplemented with NaBut. Biofilms were evaluated during adhesion, development and after maturation for metabolic activity and biomass, as well as regarding ultrastructure by scanning electron microscopy and confocal laser scanning microscopy. NaBut inhibited the growth of planktonic cells by 50% at 60 mM or 120 mM (p < 0.05) and also reduced filamentation of Trichosporon spp. NaBut reduced adhesion of Trichosporon cells by 45% (10xMIC) on average (p < 0.05). During biofilm development, NatBut (10xMIC) reduced metabolic activity and biomass up to 63% and 81%, respectively (p < 0.05). Mature biofilms were affected by NaBut (10xMIC), showing reduction of metabolic activity and biomass of approximately 48% and 77%, respectively (p < 0.05). Ultrastructure analysis showed that NaBut (MIC and 10xMIC) was able to disassemble mature biofilms. The present study describes the antifungal and anti-biofilm potential of NaBut against these opportunist emerging fungi. (Copyright © 2019. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Biofilms; Sodium butyrate; Trichosporon asahii; Trichosporon inkin |
| المشرفين على المادة: | 0 (Antifungal Agents) 107-92-6 (Butyric Acid) |
| تواريخ الأحداث: | Date Created: 20190318 Date Completed: 20190528 Latest Revision: 20190528 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.micpath.2019.03.013 |
| PMID: | 30878621 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2019.03.013 |