دورية أكاديمية
أعرض في EDS

OxyR and the hydrogen peroxide stress response in Caulobacter crescentus.

التفاصيل البيبلوغرافية
العنوان: OxyR and the hydrogen peroxide stress response in Caulobacter crescentus.
المؤلفون: Silva LG; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. Electronic address: gomess.lari@gmail.com., Lorenzetti APR; Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil. Electronic address: alan.lorenzetti@gmail.com., Ribeiro RA; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. Electronic address: rodolfo.alvarenga.ribeiro@usp.br., Alves IR; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. Electronic address: ingridreale10@gmail.com., Leaden L; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. Electronic address: leaden@usp.br., Galhardo RS; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. Electronic address: rgalhard@usp.br., Koide T; Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil. Electronic address: tkoide@fmrp.usp.br., Marques MV; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. Electronic address: mvmarque@usp.br.
المصدر: Gene [Gene] 2019 Jun 05; Vol. 700, pp. 70-84. Date of Electronic Publication: 2019 Mar 14.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
مواضيع طبية MeSH: Caulobacter crescentus/*growth & development , Gene Expression Profiling/*methods , Hydrogen Peroxide/*adverse effects , Transcription Factors/*genetics, Bacterial Proteins/genetics ; Caulobacter crescentus/drug effects ; Caulobacter crescentus/genetics ; Gene Expression Regulation, Bacterial/drug effects ; Gene Expression Regulation, Developmental/drug effects ; Gene Regulatory Networks/drug effects ; Oxidative Stress ; Sequence Analysis, RNA/methods ; Stress, Physiological
مستخلص: Oxidative stress generated by hydrogen peroxide is faced by bacteria when encountering hostile environments. In order to define the physiological and regulatory networks controlling the oxidative stress response in the free-living bacterium Caulobacter crescentus, a whole transcriptome analysis of wild type and ΔoxyR strains in the presence of hydrogen peroxide for two different exposure times was carried out. The C. crescentus response to H 2 O 2 includes a decrease of the assimilative sulfate reduction and a shift in the amino acid synthesis pathways into favoring the synthesis of histidine. Moreover, the expression of genes encoding enzymes for the depolymerization of polyhydroxybutyrate was increased, and the RpoH-dependent genes were severely repressed. Based on the expression pattern and sequence analysis, we postulate that OxyR is probably directly required for the induction of three genes (katG, ahpCF). The putative binding of OxyR to the ahpC regulatory region could be responsible for the use of one of two alternative promoters in response to oxidative stress. Nevertheless, OxyR is required for the expression of 103 genes in response to H 2 O 2 . Fur and part of its regulon were differentially expressed in response to hydrogen peroxide independently of OxyR. The non-coding RNA OsrA was upregulated in both strains, and an in silico analysis indicated that it may have a regulatory role. This work characterizes the physiological response to H 2 O 2 in C. crescentus, the regulatory networks and differentially regulated genes in oxidative stress and the participation of OxyR in this process. It is proposed that besides OxyR, a second layer of regulation may be achieved by a small regulatory RNA and other transcriptional regulators.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Caulobacter crescentus; Gene regulation; Oxidative stress; OxyR
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Transcription Factors)
BBX060AN9V (Hydrogen Peroxide)
تواريخ الأحداث: Date Created: 20190319 Date Completed: 20190513 Latest Revision: 20190513
رمز التحديث: 20231215
DOI: 10.1016/j.gene.2019.03.003
PMID: 30880241
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0038
DOI:10.1016/j.gene.2019.03.003