دورية أكاديمية
أعرض في EDS

Interplay of Chimeric Mating-Type Loci Impairs Fertility Rescue and Accounts for Intra-Strain Variability in Zygosaccharomyces rouxii Interspecies Hybrid ATCC42981.

التفاصيل البيبلوغرافية
العنوان: Interplay of Chimeric Mating-Type Loci Impairs Fertility Rescue and Accounts for Intra-Strain Variability in Zygosaccharomyces rouxii Interspecies Hybrid ATCC42981.
المؤلفون: Bizzarri M; Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy., Cassanelli S; Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy., Bartolini L; Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy., Pryszcz LP; Laboratory of Zebrafish Developmental Genomics, International Institute of Molecular and Cell Biology, Warsaw, Poland., Dušková M; Department of Membrane Transport, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia., Sychrová H; Department of Membrane Transport, Institute of Physiology, Czech Academy of Sciences, Prague, Czechia., Solieri L; Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy.
المصدر: Frontiers in genetics [Front Genet] 2019 Mar 01; Vol. 10, pp. 137. Date of Electronic Publication: 2019 Mar 01 (Print Publication: 2019).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101560621 Publication Model: eCollection Cited Medium: Print ISSN: 1664-8021 (Print) Linking ISSN: 16648021 NLM ISO Abbreviation: Front Genet Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation.
مستخلص: The pre-whole genome duplication (WGD) Zygosaccharomyces clade comprises several allodiploid strain/species with industrially interesting traits. The salt-tolerant yeast ATCC42981 is a sterile and allodiploid strain which contains two subgenomes, one of them resembling the haploid parental species Z. rouxii . Recently, different mating-type-like ( MTL ) loci repertoires were reported for ATCC42981 and the Japanese strain JCM22060, which are considered two stocks of the same strain. MTL reconstruction by direct sequencing approach is challenging due to gene redundancy, structure complexities, and allodiploid nature of ATCC42981. Here, DBG2OLC and MaSuRCA hybrid de novo assemblies of ONT and Illumina reads were combined with in vitro long PCR to definitively solve these incongruences. ATCC42981 exhibits several chimeric MTL loci resulting from reciprocal translocation between parental haplotypes and retains two MAT a/ MAT α expression loci, in contrast to MAT α in JCM22060. Consistently to these reconstructions, JCM22060, but not ATCC42981, undergoes mating and meiosis. To ascertain whether the damage of one allele at the MAT locus regains the complete sexual cycle in ATCC42981, we removed the MAT α expressed locus by gene deletion. The resulting MAT a/- hemizygous mutants did not show any evidence of sporulation, as well as of self- and out-crossing fertility, probably because incomplete silencing at the chimeric HML α cassette masks the loss of heterozygosity at the MAT locus. We also found that MAT α deletion switched off a2 transcription, an activator of a-specific genes in pre-WGD species. These findings suggest that regulatory scheme of cell identity needs to be further investigated in Z. rouxii protoploid yeast.
References: BMC Bioinformatics. 2005 Feb 15;6:31. (PMID: 15713233)
Genome Biol. 2016 Nov 25;17(1):239. (PMID: 27887629)
Genetics. 2012 May;191(1):33-64. (PMID: 22555442)
Mol Cell Biol. 1989 Nov;9(11):4613-20. (PMID: 2574822)
C R Trav Lab Carlsberg Chim. 1959;31(9):129-48. (PMID: 13639537)
Microbiol Resour Announc. 2018 Aug 2;7(4):. (PMID: 30533882)
PLoS One. 2015 Oct 22;10(10):e0140990. (PMID: 26491872)
Mol Biol Evol. 2019 Dec 1;36(12):2922-2924. (PMID: 31411700)
FEMS Yeast Res. 2017 Aug 1;17(5):. (PMID: 28899033)
Proc Biol Sci. 2002 Jun 7;269(1496):1167-71. (PMID: 12061961)
FEMS Yeast Res. 2015 Aug;15(5):fov035. (PMID: 26066552)
Fungal Genet Biol. 2008 Dec;45(12):1582-90. (PMID: 18952188)
PLoS One. 2014 Mar 18;9(3):e92203. (PMID: 24643015)
Appl Microbiol. 1967 Jul;15(4):928-34. (PMID: 6049309)
FEMS Yeast Res. 2008 Nov;8(7):996-1007. (PMID: 18355270)
Gene. 1987;57(2-3):267-72. (PMID: 3319781)
Nature. 2010 Dec 16;468(7326):959-63. (PMID: 21164485)
Mol Cell Biol. 2016 Jan 25;36(7):1164-79. (PMID: 26811328)
PLoS Biol. 2017 May 16;15(5):e2002128. (PMID: 28510588)
Curr Genet. 2004 Jun;45(6):360-70. (PMID: 15103502)
Eukaryot Cell. 2008 May;7(5):848-58. (PMID: 18375614)
Yeast. 2008 Jun;25(6):449-56. (PMID: 18509846)
Mol Biol Evol. 2005 Apr;22(4):856-73. (PMID: 15616141)
Genome Res. 2017 May;27(5):737-746. (PMID: 28100585)
Appl Microbiol Biotechnol. 2017 Jun;101(11):4617-4633. (PMID: 28396924)
Nature. 2006 Sep 28;443(7110):415-20. (PMID: 17006507)
BMC Bioinformatics. 2012 Sep 17;13:237. (PMID: 22984983)
F1000Res. 2018 Feb 5;7:. (PMID: 29568489)
PLoS One. 2016 Apr 11;11(4):e0152558. (PMID: 27065237)
Bioinformatics. 2015 Oct 1;31(19):3210-2. (PMID: 26059717)
Sci Rep. 2017 Aug 3;7(1):7213. (PMID: 28775309)
Am Nat. 2016 Dec;188(6):602-614. (PMID: 27860510)
Gigascience. 2017 Feb 1;6(2):1-13. (PMID: 28369459)
FEMS Yeast Res. 2012 Sep;12(6):703-18. (PMID: 22697168)
Proc Biol Sci. 2012 Jul 7;279(1738):2497-509. (PMID: 22492065)
Cell. 2003 Nov 14;115(4):389-99. (PMID: 14622594)
Genome Res. 2009 Oct;19(10):1696-709. (PMID: 19525356)
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20024-9. (PMID: 22123960)
Appl Environ Microbiol. 2017 Oct 17;83(21):. (PMID: 28842546)
PLoS One. 2013 Apr 16;8(4):e62121. (PMID: 23614024)
PLoS Biol. 2010 Nov 30;8(11):e1000550. (PMID: 21151344)
Nucleic Acids Res. 1996 Jul 1;24(13):2519-24. (PMID: 8692690)
BMC Genomics. 2013 Sep 14;14:623. (PMID: 24034898)
BMC Genomics. 2018 Apr 24;19(1):282. (PMID: 29690866)
Genetics. 2006 Oct;174(2):839-50. (PMID: 16951060)
Genome Announc. 2017 Feb 16;5(7):. (PMID: 28209823)
Cell. 2012 Sep 28;151(1):80-95. (PMID: 23021217)
Mol Biol Evol. 2013 Dec;30(12):2725-9. (PMID: 24132122)
FEMS Yeast Res. 2018 Nov 1;18(7):. (PMID: 30052970)
Proc Natl Acad Sci U S A. 2011 May 3;108(18):7493-8. (PMID: 21498688)
Bioinformatics. 2009 May 1;25(9):1189-91. (PMID: 19151095)
Eukaryot Cell. 2011 Sep;10(9):1183-92. (PMID: 21764908)
Biotechniques. 2011 May;50(5):325-8. (PMID: 21548894)
G3 (Bethesda). 2014 Jun 17;4(6):1011-25. (PMID: 24939186)
Nat Biotechnol. 2018 Apr;36(4):338-345. (PMID: 29431738)
Sci Rep. 2016 Aug 30;6:31900. (PMID: 27573208)
FEMS Microbiol Lett. 2018 Feb 1;365(2):. (PMID: 29228275)
Nucleic Acids Res. 2016 Jul 8;44(12):e113. (PMID: 27131372)
PLoS One. 2014 Nov 19;9(11):e112963. (PMID: 25409509)
BMC Biotechnol. 2011 Nov 17;11:109. (PMID: 22093809)
Nat Rev Genet. 2011 Nov 29;13(1):36-46. (PMID: 22124482)
Methods Mol Biol. 2014;1079:105-16. (PMID: 24170397)
Nat Genet. 2017 Jun;49(6):913-924. (PMID: 28416820)
PLoS Biol. 2015 Aug 07;13(8):e1002221. (PMID: 26252643)
Cell. 1993 Nov 5;75(3):531-41. (PMID: 8221892)
FEMS Yeast Res. 2016 Sep;16(6):. (PMID: 27493145)
Genome Res. 2017 May;27(5):787-792. (PMID: 28130360)
J Microbiol Methods. 2003 Nov;55(2):481-4. (PMID: 14529972)
Genetics. 2012 Nov;192(3):1001-14. (PMID: 22923378)
PLoS Biol. 2015 Aug 07;13(8):e1002220. (PMID: 26252497)
Genetics. 2009 Apr;181(4):1477-91. (PMID: 19171939)
Cell. 2008 Dec 12;135(6):1065-73. (PMID: 19070577)
Genetics. 2017 Jun;206(2):717-750. (PMID: 28592505)
Yeast. 2018 Jan;35(1):85-98. (PMID: 28967670)
فهرسة مساهمة: Keywords: MinION; Zygosaccharomyces; chimeric loci; interspecies hybridization; mating-type; sexual cycle; yeast
تواريخ الأحداث: Date Created: 20190319 Latest Revision: 20231006
رمز التحديث: 20231006
مُعرف محوري في PubMed: PMC6405483
DOI: 10.3389/fgene.2019.00137
PMID: 30881382
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-8021
DOI:10.3389/fgene.2019.00137