دورية أكاديمية
Contribution of YjbIH to Virulence Factor Expression and Host Colonization in Staphylococcus aureus .
| العنوان: | Contribution of YjbIH to Virulence Factor Expression and Host Colonization in Staphylococcus aureus . |
|---|---|
| المؤلفون: | Austin CM; University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, Kansas, USA., Garabaglu S; Rutgers, the State University of New Jersey, Department of Biochemistry and Microbiology, New Brunswick, New Jersey, USA., Krute CN; University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, Kansas, USA., Ridder MJ; University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, Kansas, USA., Seawell NA; University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, Kansas, USA., Markiewicz MA; University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, Kansas, USA., Boyd JM; Rutgers, the State University of New Jersey, Department of Biochemistry and Microbiology, New Brunswick, New Jersey, USA., Bose JL; University of Kansas Medical Center, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, Kansas, USA jbose@kumc.edu. |
| المصدر: | Infection and immunity [Infect Immun] 2019 May 21; Vol. 87 (6). Date of Electronic Publication: 2019 May 21 (Print Publication: 2019). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Gene Expression Regulation, Bacterial*, Bacterial Proteins/*metabolism , Oxidoreductases/*metabolism , Staphylococcal Infections/*microbiology , Staphylococcus aureus/*enzymology , Staphylococcus aureus/*growth & development , Virulence Factors/*genetics, Animals ; Bacterial Proteins/genetics ; Female ; Humans ; Metalloendopeptidases/genetics ; Metalloendopeptidases/metabolism ; Mice ; Mice, Inbred C57BL ; Operon ; Oxidoreductases/genetics ; Staphylococcus aureus/genetics ; Staphylococcus aureus/metabolism ; Virulence Factors/metabolism |
| مستخلص: | To persist within the host and cause disease, Staphylococcus aureus relies on its ability to precisely fine-tune virulence factor expression in response to rapidly changing environments. During an unbiased transposon mutant screen, we observed that disruption of a two-gene operon, yjbIH , resulted in decreased levels of pigmentation and aureolysin (Aur) activity relative to the wild-type strain. Further analyses revealed that YjbH, a predicted thioredoxin-like oxidoreductase, is predominantly responsible for the observed yjbIH mutant phenotypes, though a minor role exists for the putative truncated hemoglobin YjbI. These differences were due to significantly decreased expression of crtOPQMN and aur Previous studies found that YjbH targets the disulfide- and oxidative stress-responsive regulator Spx for degradation by ClpXP. The absence of yjbH or yjbI resulted in altered sensitivities to nitrosative and oxidative stress and iron deprivation. Additionally, aconitase activity was altered in the yjbH and yjbI mutant strains. Decreased levels of pigmentation and aureolysin (Aur) activity in the yjbH mutant were found to be Spx dependent. Lastly, we used a murine sepsis model to determine the effect of the yjbIH deletion on pathogenesis and found that the mutant was better able to colonize the kidneys and spleens during an acute infection than the wild-type strain. These studies identified changes in pigmentation and protease activity in response to YjbIH and are the first to have shown a role for these proteins during infection. (Copyright © 2019 American Society for Microbiology.) |
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| معلومات مُعتمدة: | R01 AI121073 United States AI NIAID NIH HHS; R01 AI139100 United States AI NIAID NIH HHS; R15 GM112117 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: Spx; Staphylococcus aureus; YjbH; YjbI; staphyloxanthin |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Virulence Factors) EC 1.- (Oxidoreductases) EC 3.4.24.- (Metalloendopeptidases) EC 3.4.24.29 (auR protein, Staphylococcus aureus) |
| تواريخ الأحداث: | Date Created: 20190320 Date Completed: 20191021 Latest Revision: 20200309 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6529663 |
| DOI: | 10.1128/IAI.00155-19 |
| PMID: | 30885928 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5522 |
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| DOI: | 10.1128/IAI.00155-19 |