دورية أكاديمية
أعرض في EDS

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis.

التفاصيل البيبلوغرافية
العنوان: In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis.
المؤلفون: Homa M; MTA-SZTE 'Lendület' Fungal Pathogenicity Mechanisms Research Group, Szeged, Hungary.; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary., Sándor A; MTA-SZTE 'Lendület' Fungal Pathogenicity Mechanisms Research Group, Szeged, Hungary.; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary., Tóth E; MTA-SZTE 'Lendület' Fungal Pathogenicity Mechanisms Research Group, Szeged, Hungary.; Interdisciplinary Excellence Centre, Department of Microbiology, University of Szeged, Szeged, Hungary., Szebenyi C; MTA-SZTE 'Lendület' Fungal Pathogenicity Mechanisms Research Group, Szeged, Hungary.; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.; Interdisciplinary Excellence Centre, Department of Microbiology, University of Szeged, Szeged, Hungary., Nagy G; MTA-SZTE 'Lendület' Fungal Pathogenicity Mechanisms Research Group, Szeged, Hungary.; Interdisciplinary Excellence Centre, Department of Microbiology, University of Szeged, Szeged, Hungary., Vágvölgyi C; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.; Interdisciplinary Excellence Centre, Department of Microbiology, University of Szeged, Szeged, Hungary., Papp T; MTA-SZTE 'Lendület' Fungal Pathogenicity Mechanisms Research Group, Szeged, Hungary.; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.; Interdisciplinary Excellence Centre, Department of Microbiology, University of Szeged, Szeged, Hungary.
المصدر: Frontiers in microbiology [Front Microbiol] 2019 Mar 06; Vol. 10, pp. 441. Date of Electronic Publication: 2019 Mar 06 (Print Publication: 2019).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Members of the Scedosporium apiospermum species complex are the second most frequently isolated pathogens after Aspergillus fumigatus from cystic fibrosis (CF) patients with fungal pulmonary infections. Even so, the main risk factors for the infection are unrevealed. According to previous studies, bacterial infections might reduce the risk of a fungal infection, but an antibacterial therapy may contribute to the airway colonization by several fungal pathogens. Furthermore, corticosteroids, which are often used to reduce lung inflammation in children and adults with CF, are also proved to enhance the growth of A. fumigatus in vitro . Considering all the above discussed points, we aimed to test how Pseudomonas aeruginosa influences the growth of scedosporia and to investigate the potential effect of commonly applied antibacterial agents and corticosteroids on Scedosporium species. Direct interactions between fungal and bacterial strains were tested using the disk inhibition method. Indirect interactions via volatile compounds were investigated by the plate-in-plate method, while the effect of bacterial media-soluble molecules was tested using a modified cellophane assay and also in liquid culture media conditioned by P. aeruginosa . To test the effect of bacterial signal molecules, antibacterial agents and corticosteroids on the fungal growth, the broth microdilution method was used. We also investigated the germination ability of Scedosporium conidia in the presence of pyocyanin and diffusible signal factor by microscopy. According to our results, P. aeruginosa either inhibited or enhanced the growth of scedosporia depending on the culture conditions and the mode of interactions. When the two pathogens were cultured physically separately from each other in the plate-in-plate tests, the presence of the bacteria was able to stimulate the growth of several fungal isolates. While in direct physical contact, bacterial strains inhibited the fungal growth. This effect might be attributed to bacterial signal molecules, which also proved to inhibit the germination and growth of scedosporia. In addition, antibacterial agents showed growth-promoting, while corticosteroids exhibited growth inhibitory effect on several Scedosporium isolates. These data raise the possibility that a P. aeruginosa infection or a previously administered antibacterial therapy might be able to increase the chance of a Scedosporium colonization in a CF lung.
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فهرسة مساهمة: Keywords: Pseudomonas aeruginosa; Scedosporium; cystic fibrosis; diffusible signal factor; flucloxacillin; pyocyanin; tobramycin; volatile organic compounds
تواريخ الأحداث: Date Created: 20190322 Latest Revision: 20201001
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6414507
DOI: 10.3389/fmicb.2019.00441
PMID: 30894846
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2019.00441