دورية أكاديمية
Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse.
| العنوان: | Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse. |
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| المؤلفون: | Korrell KV; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Disser J; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.; Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Parley K; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Vadisiute A; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Requena-Komuro MC; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Fodder H; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Pollart C; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.; Institute of Molecular Biology and Medicine (IBMM), Université Libre de Bruxelles (ULB), Gosselies, Belgium., Knott G; EPFL SV PTECH PTBIOEM, AI 0143 (Bâtiment AI), Lausanne, Switzerland., Molnár Z; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK., Hoerder-Suabedissen A; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK. |
| المصدر: | Journal of anatomy [J Anat] 2019 Sep; Vol. 235 (3), pp. 452-467. Date of Electronic Publication: 2019 Mar 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Publishing Country of Publication: England NLM ID: 0137162 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-7580 (Electronic) Linking ISSN: 00218782 NLM ISO Abbreviation: J Anat Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2002- : Oxford : Blackwell Publishing Original Publication: London, Cambridge Univ. Press [etc.]. |
| مواضيع طبية MeSH: | Cerebral Cortex/*growth & development , Myelin Sheath/*metabolism, Animals ; Female ; Mice, Inbred C57BL ; Mice, Transgenic ; Myelin Sheath/ultrastructure ; Pregnancy |
| مستخلص: | Myelination of axons by oligodendrocytes in the central nervous system is crucial for fast, saltatory conduction of action potentials. As myelination is central for brain development and plasticity, and deficits are implicated in several neural disorders such as multiple sclerosis, major depressive disorder, bipolar disorder and schizophrenia, it is important to elucidate the underlying mechanisms regulating myelination. Numerous mechanisms have been proposed by which the communication between oligodendrocytes and active axons may regulate the onset and maintenance of activity-dependent myelination. We compared two models of 'silencing' layer V and/or VI cortical projection neurons from early stages by either decreasing their excitability through Kir2.1 expression, an inward rectifying potassium channel, introduced through in utero electroporation at embryonic day (E)13.5, or inhibiting regulated vesicular release through Cre-dependent knock-out of synaptosomal associated protein 25 kDA (SNAP25). SNAP25 is a component of the soluble N-ethylmaleimide fusion protein attachment protein receptor (SNARE) complex, which, among others, is needed for calcium-dependent regulated vesicle release from synapses. In layer VI cortical projection neurons in the Ntsr1-Cre;Ai14;Snap25 fl/fl mouse, we found that inhibiting regulated vesicular release significantly decreased the amount of myelin basic protein (MBP, used as marker for myelination) and the amount of myelinated projections at postnatal day (P)14 without affecting the initial timing of onset of myelination in the brain (at P7/P8). Additionally, overall oligodendrocyte maturation appears to be affected. A strong trend towards reduced node of Ranvier (NoR) length was also observed in Ntsr1-Cre;Ai14;Snap25 fl/fl corpus callosum. An equally strong trend towards reduced NoR length was observed in Rbp4-Cre;Ai14;Snap25 fl/fl corpus callosum at P14, and the g-ratio in the spinal cord dorsal column was reduced at P18. However, no measurable differences in levels of MBP were detected in the striatum when comparing Rbp4-Cre;Ai14;Snap25 fl/fl and control brains. Conversely, Kir2.1 in utero electroporation at E13.5 did not significantly affect the amount of MBP or number of myelinated callosal axons at P14 but did significantly decrease the NoR length measured in the corpus callosum. It therefore seems likely that the excitability of the neuron can potentially perform a modulating function of myelin characteristics, whereas regulated vesicular release has the potential to have a more pronounced effect on overall myelination, but in a cell-type specific manner. (© 2019 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.) |
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| معلومات مُعتمدة: | International Anatomical Society; BB/F003285/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; G0700377 United Kingdom MRC_ Medical Research Council; G00900901 United Kingdom MRC_ Medical Research Council; G0300200 United Kingdom MRC_ Medical Research Council; G0900901 United Kingdom MRC_ Medical Research Council; MR/N026039/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: Kir2.1; Node of Ranvier; Ntsr1-Cre; Rbp4-Cre; SNAP25; layer VI and V projection neurons; myelination |
| تواريخ الأحداث: | Date Created: 20190323 Date Completed: 20200911 Latest Revision: 20211006 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6704270 |
| DOI: | 10.1111/joa.12974 |
| PMID: | 30901089 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1469-7580 |
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| DOI: | 10.1111/joa.12974 |