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Proteomics and microscopy tools for the study of antimicrobial resistance and germination mechanisms of bacterial spores.

التفاصيل البيبلوغرافية
العنوان: Proteomics and microscopy tools for the study of antimicrobial resistance and germination mechanisms of bacterial spores.
المؤلفون: Abhyankar WR; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Wen J; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands; Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Swarge BN; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Tu Z; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., de Boer R; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Smelt JPPM; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., de Koning LJ; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Manders E; Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., de Koster CG; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Brul S; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands. Electronic address: s.brul@uva.nl.
المصدر: Food microbiology [Food Microbiol] 2019 Aug; Vol. 81, pp. 89-96. Date of Electronic Publication: 2018 Mar 14.
نوع المنشور: Journal Article; Review
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 8601127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9998 (Electronic) Linking ISSN: 07400020 NLM ISO Abbreviation: Food Microbiol Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Elsevier
Original Publication: London ; Orlando : Academic Press, c1984-
مواضيع طبية MeSH: Drug Resistance, Bacterial* , Food Microbiology*, Microscopy/*methods , Proteomics/*methods , Spores, Bacterial/*growth & development , Spores, Bacterial/*metabolism, Bacillus subtilis/genetics ; Bacillus subtilis/growth & development ; Bacillus subtilis/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Food Handling ; Food Preservation ; Genetic Heterogeneity ; Hydrogen-Ion Concentration ; Kinetics ; Models, Theoretical ; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics ; Promoter Regions, Genetic ; Protein Kinases/genetics ; Protein Kinases/metabolism ; Spores, Bacterial/cytology ; Spores, Bacterial/genetics ; Stress, Physiological
مستخلص: Bacterial spores are ubiquitous in nature and can withstand both chemical and physical stresses. Spores can survive food preservation processes and upon outgrowth cause food spoilage as well as safety risks. The heterogeneous germination and outgrowth behavior of isogenic spore populations exacerbates this risk. A major unknown factor of spores is likely to be the inherently heterogeneous spore protein composition. The proteomics methods discussed here help in broadening the knowledge about spore structure and identification of putative target proteins from spores of different spore formers. Approaches to synchronize Bacillus subtilis spore formation, and to analyze spore proteins as well as the physiology of spore germination and outgrowth are also discussed. Live-imaging and fluorescence microscopy techniques discussed here allow analysis, at single cell level, of the 'germinosome', the process of spore germination itself, spore outgrowth and the spore intracellular pH dynamics. For the latter, a recently published improved pHluorin (IpHluorin) under control of the ptsG promoter is applicable. While the data obtained from such tools offers novel insight in the mechanisms of bacterial spore awakening, it may also be used to probe candidate antimicrobial compounds for inhibitory effects on spore germination and strengthen microbial risk assessment.
(Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
فهرسة مساهمة: Keywords: Bacterial spores; Heterogeneity; Live cell imaging; Proteomics; Stress resistance
المشرفين على المادة: 0 (Bacterial Proteins)
0 (kinA protein, Bacillus subtilis)
EC 2.7.- (Protein Kinases)
EC 2.7.1.- (Phosphoenolpyruvate Sugar Phosphotransferase System)
EC 2.7.1.- (phosphoenolpyruvate-glucose phosphotransferase)
تواريخ الأحداث: Date Created: 20190327 Date Completed: 20190503 Latest Revision: 20190503
رمز التحديث: 20231215
DOI: 10.1016/j.fm.2018.03.006
PMID: 30910091
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-9998
DOI:10.1016/j.fm.2018.03.006