دورية أكاديمية
A Human Hematopoietic Niche Model Supporting Hematopoietic Stem and Progenitor Cells In Vitro.
| العنوان: | A Human Hematopoietic Niche Model Supporting Hematopoietic Stem and Progenitor Cells In Vitro. |
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| المؤلفون: | Braham MVJ; Department of Orthopaedics, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.; Regenerative Medicine Center, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands., Li Yim ASP; Department of Orthopaedics, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.; Regenerative Medicine Center, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands., Garcia Mateos J; Department of Orthopaedics, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.; Regenerative Medicine Center, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands., Minnema MC; Department of Hematology, University Medical Center Utrecht Cancer Center, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands., Dhert WJA; Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL, Utrecht, The Netherlands., Öner FC; Department of Orthopaedics, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands., Robin C; Regenerative Medicine Center, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.; Hubrecht Institute-KNAW, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands., Alblas J; Department of Orthopaedics, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands.; Regenerative Medicine Center, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands. |
| المصدر: | Advanced healthcare materials [Adv Healthc Mater] 2019 May; Vol. 8 (10), pp. e1801444. Date of Electronic Publication: 2019 Apr 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101581613 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2192-2659 (Electronic) Linking ISSN: 21922640 NLM ISO Abbreviation: Adv Healthc Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim : Wiley-VCH, 2012- |
| مواضيع طبية MeSH: | Hematopoietic Stem Cells/*cytology , Hydrogels/*chemistry, Adipogenesis/drug effects ; Alginates/chemistry ; Antigens, CD34/metabolism ; Cell Culture Techniques ; Cell Differentiation/drug effects ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Collagen/chemistry ; Drug Combinations ; Endothelial Cells/cytology ; Endothelial Cells/metabolism ; Hematopoietic Stem Cells/metabolism ; Humans ; Hydrogels/pharmacology ; Laminin/chemistry ; Osteogenesis/drug effects ; Proteoglycans/chemistry ; Stem Cell Niche |
| مستخلص: | Niches in the bone marrow regulate hematopoietic stem and progenitor cell (HSPC) fate and behavior through cell-cell interactions and soluble factor secretion. The niche-HSPC crosstalk is a very complex process not completely elucidated yet. To aid further investigation of this crosstalk, a functional in vitro 3D model that closely represents the main supportive compartments of the bone marrow is developed. Different combinations of human stromal cells and hydrogels are tested for their potential to maintain CD34 + HSPCs. Cell viability, clonogenic hematopoietic potential, and surface marker expression are assessed over time. Optimal HSPC support is obtained in presence of adipogenic and osteogenic cells, together with progenitor derived endothelial cells. When cultured in a bioactive hydrogel, the supportive cells self-assemble into a hypoxic stromal network, stimulating CD34 + CD38 + cell formation, while maintaining the pool of CD34 + 38 - HSPCs. HSPC clusters colocalize with the stromal networks, in close proximity to sinusoidal clusters of CD31 + endothelial cells. Importantly, the primary in vitro niche model supports HSPCs with no cytokine addition. Overall, the engineered primary 3D bone marrow environment provides an easy and reliable model to further investigate interactions between HSPCs and their endosteal and perivascular niches, in the context of normal hematopoiesis or blood-related diseases. (© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| معلومات مُعتمدة: | 2013 International Utrecht University |
| فهرسة مساهمة: | Keywords: 3D in vitro models; bone marrow microenvironments; endosteal niches; hematopoietic stem cell maintenance; perivascular niches |
| المشرفين على المادة: | 0 (Alginates) 0 (Antigens, CD34) 0 (Drug Combinations) 0 (Hydrogels) 0 (Laminin) 0 (Proteoglycans) 119978-18-6 (matrigel) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20190404 Date Completed: 20200707 Latest Revision: 20200707 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/adhm.201801444 |
| PMID: | 30941927 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2192-2659 |
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| DOI: | 10.1002/adhm.201801444 |