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Cytokines secreted by human Wharton's jelly stem cells inhibit the proliferation of ovarian cancer (OVCAR3) cells in vitro .

التفاصيل البيبلوغرافية
العنوان: Cytokines secreted by human Wharton's jelly stem cells inhibit the proliferation of ovarian cancer (OVCAR3) cells in vitro .
المؤلفون: Kalamegam G; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.; Faculty of Medicine, AIMST University, Bedong, Kedah 08100, Malaysia., Sait KHW; Department of Obstetrics and Gynaecology, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah 22252, Kingdom of Saudi Arabia., Anfinan N; Department of Obstetrics and Gynaecology, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah 22252, Kingdom of Saudi Arabia., Kadam R; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia., Ahmed F; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia., Rasool M; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia., Naseer MI; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia., Pushparaj PN; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia., Al-Qahtani M; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.
المصدر: Oncology letters [Oncol Lett] 2019 May; Vol. 17 (5), pp. 4521-4531. Date of Electronic Publication: 2019 Mar 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Spandidos Publications] Country of Publication: Greece NLM ID: 101531236 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1792-1074 (Print) Linking ISSN: 17921074 NLM ISO Abbreviation: Oncol Lett Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Athens : Spandidos Publications]
مستخلص: Cytokines enhance tumour cell recognition via cytotoxic effector cells and are therefore effectively used in cancer immunotherapy. Mesenchymal stem cells have efficient homing potential and have been used to target and inhibit various types of cancer mediated by the release of soluble/bioactive factors. Initial evaluation of the human Wharton's jelly stem cell conditioned medium (hWJSC-CM) and cell lysate (hWJSC-CL) against an ovarian cancer cell line (OVCAR3) demonstrated their inhibitory effect in vitro . The secreted cytokine profile was then studied to understand whether the OVCAR3 inhibitory effect was mediated by the cytokines. Expression of cytokines in OVCAR3 following 48 h treatment with hWJSC extracts, namely the hWJSC-CM (50%) and hWJSC-CL (10 µg/ml), was evaluated using multiplex cytokine assay. Paclitaxel (5 nM) was used as a positive control. Cytokines tumour necrosis factor α, interleukin (IL)-4, IL-6, IL-8, IL-10, IL-13, IL-17, IL-1β and granulocyte colony-stimulating factor, reported to be involved in tumour growth, invasion and migration, were significantly decreased. Cytokines with antitumour effects, namely IL-1 receptor antagonist (IL-1RA), IL-2, IL-2 receptor, IL-5, IL-7, IL-12, IL-15, interferon (IFN)-α and IFN-γ, were mildly increased or decreased. Only the increases in IL-1RA (with paclitaxel, hWJSC-CM and hWJSC-CL) and granulocyte-macrophage colony-stimulating factor (with hWJSC-CL) were statistically significant. The chemokines monocyte chemoattractant protein 1, macrophage inflammatory protein (MIP)-1α, MIP-1β and Regulated Upon Activation, Normally T-Expressed, and Secreted were significantly decreased while monokine induced by IFN-γ, IFN-γ induced protein 10 and Eotaxin demonstrated mild decreases. The growth factors basic fibroblast growth factor, vascular endothelial growth factor and hepatocyte growth factor were significantly decreased. Heatmaps demonstrated differential fold changes in cytokines and hierarchical cluster analysis revealed 3 major and 7 minor sub-clusters of associated cytokines, chemokines and growth factors. In conclusion, the hWJSC extracts decreased the expression of oncogenic cytokines, chemokines and growth factors, which mediated the inhibition of OVCAR3 cells in vitro .
References: Semin Surg Oncol. 2000 Jul-Aug;19(1):3-10. (PMID: 10883018)
Bioinformatics. 2002 Jan;18(1):207-8. (PMID: 11836235)
N Engl J Med. 2003 Jan 16;348(3):203-13. (PMID: 12529460)
Oncology. 2005;68(2-3):138-45. (PMID: 16006751)
Anticancer Res. 2006 Mar-Apr;26(2C):1715-8. (PMID: 16617566)
J Transl Med. 2006 Nov 10;4:48. (PMID: 17096856)
Hepatology. 2007 Mar;45(3):746-54. (PMID: 17326190)
J Huazhong Univ Sci Technolog Med Sci. 2007 Dec;27(6):721-4. (PMID: 18231753)
Nature. 2008 Jul 17;454(7202):345-9. (PMID: 18528331)
Anticancer Res. 2009 Aug;29(8):2875-84. (PMID: 19661290)
J Clin Invest. 2009 Oct;119(10):3011-23. (PMID: 19741298)
Cancer Res. 2009 Nov 15;69(22):8678-85. (PMID: 19887609)
Clin Cancer Res. 2009 Dec 15;15(24):7744-7748. (PMID: 19996221)
J Oncol. 2010;2010:426956. (PMID: 20049170)
CA Cancer J Clin. 2010 Sep-Oct;60(5):277-300. (PMID: 20610543)
Reprod Biomed Online. 2010 Sep;21(3):391-401. (PMID: 20638335)
Stem Cell Rev. 2010 Dec;6(4):665-76. (PMID: 20711690)
Biol Chem. 2010 Aug;391(8):937-45. (PMID: 20731543)
J Cell Biochem. 2012 Jun;113(6):2027-39. (PMID: 22275115)
Adv Biochem Eng Biotechnol. 2013;130:209-66. (PMID: 22990585)
Immunol Rev. 2012 Nov;250(1):277-302. (PMID: 23046136)
J Clin Invest. 2013 Sep;123(9):3740-50. (PMID: 23945238)
Stem Cells Int. 2013;2013:130763. (PMID: 24194766)
Eur Cytokine Netw. 2013 Jul-Sep;24(3):106-13. (PMID: 24197277)
Br J Cancer. 2014 Jan 7;110(1):133-45. (PMID: 24220695)
Tumour Biol. 2014 Oct;35(10):10539-46. (PMID: 25060177)
Clin Cancer Res. 2015 Jun 15;21(12):2840-50. (PMID: 25712684)
Int J Mol Sci. 2015 May 06;16(5):10267-80. (PMID: 25955647)
Sci Transl Med. 2015 Oct 28;7(311):311ra170. (PMID: 26511507)
Int J Mol Sci. 2015 Dec 16;16(12):30015-33. (PMID: 26694366)
Cytokine Growth Factor Rev. 2016 Jun;29:109-15. (PMID: 27026228)
Front Physiol. 2016 May 24;7:180. (PMID: 27252654)
Br J Cancer. 2016 Aug 23;115(5):553-63. (PMID: 27490802)
Clin Med Insights Oncol. 2016 Aug 02;10:71-6. (PMID: 27512342)
CA Cancer J Clin. 2017 Jan;67(1):7-30. (PMID: 28055103)
J Cancer. 2017 Jan 1;8(1):85-96. (PMID: 28123601)
Oncol Lett. 2017 May;13(5):2855-2866. (PMID: 28521391)
Cancer Sci. 2017 Oct;108(10):1939-1946. (PMID: 28756624)
Cancer Lett. 2018 Feb 1;414:239-249. (PMID: 29175461)
Front Immunol. 2018 Apr 09;9:586. (PMID: 29686666)
Cancer Immunol Immunother. 2018 Oct;67(10):1519-1531. (PMID: 30039427)
Am J Epidemiol. 1994 Apr 1;139(7):654-61. (PMID: 8166126)
فهرسة مساهمة: Keywords: chemokines; cluster analysis; cytokines; growth factors; ovarian cancer; stem cells
تواريخ الأحداث: Date Created: 20190405 Latest Revision: 20200930
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6444458
DOI: 10.3892/ol.2019.10094
PMID: 30944641
قاعدة البيانات: MEDLINE
الوصف
تدمد:1792-1074
DOI:10.3892/ol.2019.10094