دورية أكاديمية
أعرض في EDS

Effects of Low Protein Diet on Nuclear Factor Erythroid 2-Related Factor 2 Gene Expression in Nondialysis Chronic Kidney Disease Patients.

التفاصيل البيبلوغرافية
العنوان: Effects of Low Protein Diet on Nuclear Factor Erythroid 2-Related Factor 2 Gene Expression in Nondialysis Chronic Kidney Disease Patients.
المؤلفون: Anjos JSD; Graduate Program in Cardiovascular Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil. Electronic address: ju_saraiva@me.com., Cardozo LFMF; Graduate Program in Cardiovascular Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil., Black AP; Graduate Program in Medical Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil., Santos da Silva G; Graduate Program in Nutrition Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil., Vargas Reis DCM; Graduate Program in Medical Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil., Salarolli R; Graduate Program in Nutrition Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil., Carraro-Eduardo JC; Medicine Faculty, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil., Mafra D; Graduate Program in Cardiovascular Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil; Graduate Program in Medical Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil; Graduate Program in Nutrition Sciences, Fluminense Federal University (UFF), Niterói, Rio de Janeiro, Brazil.
المصدر: Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation [J Ren Nutr] 2020 Jan; Vol. 30 (1), pp. 46-52. Date of Electronic Publication: 2019 Apr 05.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: W.B. Saunders Co Country of Publication: United States NLM ID: 9112938 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-8503 (Electronic) Linking ISSN: 10512276 NLM ISO Abbreviation: J Ren Nutr Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Philadelphia, PA : W.B. Saunders Co., [1991-
مواضيع طبية MeSH: Diet, Protein-Restricted/*methods , Gene Expression/*genetics , NF-E2-Related Factor 2/*blood , NF-E2-Related Factor 2/*genetics , Renal Insufficiency, Chronic/*blood , Renal Insufficiency, Chronic/*genetics, Female ; Humans ; Longitudinal Studies ; Male ; Middle Aged
مستخلص: Objective(s): Low protein diets (LPD; 0.6 g/kg/day), prescribed for nondialysis chronic kidney disease (CKD) patients, have demonstrated numerous benefits. LPDs may modulate inflammation and oxidative stress through the nuclear factor erythroid 2-related factor 2 (Nrf2), which encodes antioxidant and phase II detoxifying enzymes. LPDs also inhibit or antagonize nuclear factor kB (NF-kB) activity, which orchestrates inflammatory and oxidative stress responses. The objective of this study was to evaluate the effects of LPD on Nfr2 and NF-κB messenger RNA (mRNA) expression in nondialysis CKD patients.
Methods: In this longitudinal study, a LPD was prescribed for 30 nondialysis CKD patients for 6 months. Peripheral blood mononuclear cells were isolated, and quantitative real-time polymerase chain reaction analysis was performed to evaluate Nrf2, NF-κB, and NADPH quinine oxidoreductase-1 mRNA expression. Thiobarbituric acid-reactive substance (TBARS) levels, a marker of lipid peroxidation, were also evaluated.
Results: (Age 55.5 ± 14.0 years; body mass index 29.1 ± 5.9 kg/m 2 ; glomerular filtration rate 35.6 ± 12.2 mL/minute). After 6 months of nutritional intervention, Nrf2 mRNA expression increased from 0.85 (0.47-1.56) to 1.28 (0.63-2.63) nmol/mL (P = .03), and TBARS levels were significantly decreased from 1.78 (1.31-2.38) to 1.30 (1.07-2.22) nmol/mL (P = .04). NF-κB mRNA expression showed no significant difference after 6 months, but the Nrf2/NF-κB ratio was increased.
Conclusion(s): In this study, a LPD appeared to modulate Nrf2 expression and decrease the levels of TBARS in nondialysis CKD patients. However, more studies are needed to confirm the effectiveness of LPD on the modulation of transcription factors involved with oxidative stress and inflammation in nondialysis CKD patients.
(Copyright © 2019 National Kidney Foundation, Inc. Published by Elsevier Inc. All rights reserved.)
المشرفين على المادة: 0 (NF-E2-Related Factor 2)
تواريخ الأحداث: Date Created: 20190409 Date Completed: 20210609 Latest Revision: 20210609
رمز التحديث: 20231215
DOI: 10.1053/j.jrn.2019.01.005
PMID: 30956090
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-8503
DOI:10.1053/j.jrn.2019.01.005