دورية أكاديمية
أعرض في EDS

Spatiotemporal control of coacervate formation within liposomes.

التفاصيل البيبلوغرافية
العنوان: Spatiotemporal control of coacervate formation within liposomes.
المؤلفون: Deshpande S; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Brandenburg F; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Lau A; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Last MGF; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Spoelstra WK; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Reese L; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Wunnava S; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Dogterom M; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Dekker C; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands. c.dekker@tudelft.nl.
المصدر: Nature communications [Nat Commun] 2019 Apr 17; Vol. 10 (1), pp. 1800. Date of Electronic Publication: 2019 Apr 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Cell Membrane Permeability*, Artificial Cells/*metabolism , Liposomes/*metabolism , Microfluidics/*methods, Artificial Cells/chemistry ; High-Throughput Screening Assays/instrumentation ; High-Throughput Screening Assays/methods ; Lab-On-A-Chip Devices ; Liposomes/chemistry ; Microfluidics/instrumentation ; Phase Transition
مستخلص: Liquid-liquid phase separation (LLPS), especially coacervation, plays a crucial role in cell biology, as it forms numerous membraneless organelles in cells. Coacervates play an indispensable role in regulating intracellular biochemistry, and their dysfunction is associated with several diseases. Understanding of the LLPS dynamics would greatly benefit from controlled in vitro assays that mimic cells. Here, we use a microfluidics-based methodology to form coacervates inside cell-sized (~10 µm) liposomes, allowing control over the dynamics. Protein-pore-mediated permeation of small molecules into liposomes triggers LLPS passively or via active mechanisms like enzymatic polymerization of nucleic acids. We demonstrate sequestration of proteins (FtsZ) and supramolecular assemblies (lipid vesicles), as well as the possibility to host metabolic reactions (β-galactosidase activity) inside coacervates. This coacervate-in-liposome platform provides a versatile tool to understand intracellular phase behavior, and these hybrid systems will allow engineering complex pathways to reconstitute cellular functions and facilitate bottom-up creation of synthetic cells.
التعليقات: Comment in: Nat Methods. 2019 Jun;16(6):459. (PMID: 31147646)
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المشرفين على المادة: 0 (Liposomes)
تواريخ الأحداث: Date Created: 20190419 Date Completed: 20190524 Latest Revision: 20210109
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6470218
DOI: 10.1038/s41467-019-09855-x
PMID: 30996302
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-019-09855-x