دورية أكاديمية
أعرض في EDS

Optimizing PCR Detection of West Nile Virus from Body Fluid Specimens to Delineate Natural History in an Infected Human Cohort.

التفاصيل البيبلوغرافية
العنوان: Optimizing PCR Detection of West Nile Virus from Body Fluid Specimens to Delineate Natural History in an Infected Human Cohort.
المؤلفون: Gorchakov R; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. rodion.gorchakov@gmail.com., Gulas-Wroblewski BE; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. Bonnie.Gulas-Wroblewski@bcm.edu.; Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX 77843, USA. Bonnie.Gulas-Wroblewski@bcm.edu., Ronca SE; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. ronca@bcm.edu., Ruff JC; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. jruff@bcm.edu., Nolan MS; University of South Carolina, Arnold School of Public Health, Columbia, SC 29208, USA. MSNOLAN@mailbox.sc.edu., Berry R; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. rberry@bcm.edu., Alvarado RE; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. Rojelio.Alvarado@bcm.edu., Gunter SM; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. sm22@bcm.edu., Murray KO; Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine and Texas Children's Hospital, Houston, TX 77030, USA. kmurray@bcm.edu.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2019 Apr 19; Vol. 20 (8). Date of Electronic Publication: 2019 Apr 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Body Fluids/*virology , Polymerase Chain Reaction/*methods , West Nile Fever/*virology , West Nile virus/*isolation & purification, Cohort Studies ; Humans ; Male ; RNA, Viral/isolation & purification ; Saliva/virology ; Semen/virology ; West Nile Fever/blood ; West Nile Fever/urine
مستخلص: West Nile virus (WNV), a mosquito-borne arbovirus, remains a major global health concern. In this study, we optimized PCR methods then assessed serially-collected whole blood (WB), urine (UR), saliva, and semen specimens from a large cohort of WNV-positive participants to evaluate the natural history of infection and persistent shedding of WNV RNA. Viral RNA extraction protocols for frozen WB and UR specimens were optimized and validated through spiking experiments to maximize recovery of viral RNA from archived specimens and to assess the degradation of WNV RNA in stored UR specimens. The resultant procedures were used in conjunction with PCR detection to identify WNV-positive specimens and to quantify their viral loads. A total of 59 of 352 WB, 10 of 38 UR, and 2 of 34 saliva specimens tested positive for WNV RNA. Although a single semen specimen was positive 22 days post onset, we could not definitively confirm the presence of WNV RNA in the remaining specimens. WNV RNA-positive UR specimens exhibited profound loss of viral RNA during storage, highlighting the need for optimal preservation pre-storage. This study provides optimized methods for WNV RNA detection among different fluid types and offers alternative options for diagnostic testing during the acute stages of WNV.
References: Lancet Infect Dis. 2002 Sep;2(9):519-29. (PMID: 12206968)
Lancet Infect Dis. 2004 Sep;4(9):547-56. (PMID: 15336221)
Am J Trop Med Hyg. 2005 Mar;72(3):320-4. (PMID: 15772329)
J Infect Dis. 2005 Jul 15;192(2):287-95. (PMID: 15962223)
J Vet Diagn Invest. 2005 Nov;17(6):574-8. (PMID: 16475517)
Clin Infect Dis. 2006 Sep 15;43(6):723-30. (PMID: 16912946)
Clin Infect Dis. 2007 Jun 15;44(12):1617-24. (PMID: 17516407)
J Clin Microbiol. 2007 Aug;45(8):2480-5. (PMID: 17522268)
Clin Infect Dis. 2007 Jul 15;45(2):181-6. (PMID: 17578776)
J Gen Virol. 2008 Dec;89(Pt 12):3073-9. (PMID: 19008395)
J Clin Exp Neuropsychol. 2010 Jan;32(1):81-7. (PMID: 19513920)
J Infect Dis. 2010 Jan 1;201(1):2-4. (PMID: 19961306)
Uirusu. 1977 Jun;27(1):21-6. (PMID: 203101)
PLoS One. 2010 May 14;5(5):e10649. (PMID: 20498839)
J Infect Dis. 2011 Feb 1;203(3):344-7. (PMID: 21208926)
Epidemiol Infect. 2011 Jun;139(6):807-17. (PMID: 21342610)
J Infect Dis. 2012 May 1;205(9):1476-7. (PMID: 22438324)
PLoS One. 2012;7(7):e40374. (PMID: 22792293)
PLoS Negl Trop Dis. 2013 Jun 13;7(6):e2275. (PMID: 23785537)
J Infect Dis. 2013 Oct 1;208(7):1086-92. (PMID: 23821721)
PLoS Negl Trop Dis. 2013 Jul 11;7(7):e2311. (PMID: 23875046)
J Clin Psychol. 2012 Jul;68(7):801-8. (PMID: 23929558)
Am J Trop Med Hyg. 2014 Mar;90(3):389-390. (PMID: 24515938)
Transfusion. 2014 Dec;54(12):3232-41. (PMID: 24965017)
PLoS One. 2014 Jul 23;9(7):e102953. (PMID: 25054656)
Viral Immunol. 2014 Sep;27(7):327-33. (PMID: 25062274)
J Clin Virol. 2014 Nov;61(3):353-8. (PMID: 25242312)
J Biomol Tech. 2014 Dec;25(4):96-110. (PMID: 25365790)
Microbes Infect. 2015 Feb;17(2):163-8. (PMID: 25499188)
Am J Trop Med Hyg. 2015 May;92(5):1006-1012. (PMID: 25802426)
J Clin Virol. 2015 Jul;68:53-5. (PMID: 26071336)
PLoS Negl Trop Dis. 2015 Sep 25;9(9):e0004100. (PMID: 26406240)
Sci Rep. 2016 Jan 20;6:19529. (PMID: 26787294)
PLoS One. 2016 Mar 07;11(3):e0148898. (PMID: 26950214)
Euro Surveill. 2016;21(10):30159. (PMID: 26987769)
Emerg Infect Dis. 2016 Sep;22(9):1647-9. (PMID: 27322782)
J Clin Microbiol. 2016 Sep;54(9):2294-7. (PMID: 27335150)
Euro Surveill. 2016 Jun 30;21(26):. (PMID: 27386894)
Emerg Infect Dis. 2017 Jan;23(1):99-101. (PMID: 27748649)
MMWR Morb Mortal Wkly Rep. 2017 Jan 20;66(2):51-55. (PMID: 28103209)
Muscle Nerve. 2018 Jan;57(1):77-82. (PMID: 28380696)
J Virol Methods. 2017 Oct;248:66-70. (PMID: 28472623)
Orv Hetil. 2017 May;158(20):791-796. (PMID: 28502209)
Am J Trop Med Hyg. 2017 Dec;97(6):1913-1919. (PMID: 29141749)
Am J Trop Med Hyg. 2019 Feb;100(2):427-433. (PMID: 30560770)
Nat Rev Urol. 2019 Apr;16(4):211-230. (PMID: 30696994)
Arch Virol. 1983;75(1-2):71-86. (PMID: 6299247)
معلومات مُعتمدة: N/A The Chao Foundation
فهرسة مساهمة: Keywords: Houston West Nile cohort; PCR; West Nile virus; prolonged detection; saliva; semen; urine; virus shedding; whole blood
المشرفين على المادة: 0 (RNA, Viral)
تواريخ الأحداث: Date Created: 20190424 Date Completed: 20190807 Latest Revision: 20200225
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6514913
DOI: 10.3390/ijms20081934
PMID: 31010160
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms20081934