دورية أكاديمية
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Functional Analysis of Coilin in Virus Resistance and Stress Tolerance of Potato Solanum tuberosum using CRISPR-Cas9 Editing.

التفاصيل البيبلوغرافية
العنوان: Functional Analysis of Coilin in Virus Resistance and Stress Tolerance of Potato Solanum tuberosum using CRISPR-Cas9 Editing.
المؤلفون: Makhotenko AV; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia.; Moscow State University, 119234, Moscow, Russia., Khromov AV; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia.; Moscow State University, 119234, Moscow, Russia., Snigir EA; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia., Makarova SS; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia.; Moscow State University, 119234, Moscow, Russia., Makarov VV; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia., Suprunova TP; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia., Kalinina NO; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia. kalinina@genebee.msu.ru.; Moscow State University, 119234, Moscow, Russia. kalinina@genebee.msu.ru., Taliansky ME; Doka Gene Technologies Ltd, 141880, Rogachevo, Moscow oblast, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia.
المصدر: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2019 May; Vol. 484 (1), pp. 88-91. Date of Electronic Publication: 2019 Apr 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pleiades Publishing Country of Publication: United States NLM ID: 101126895 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1608-3091 (Electronic) Linking ISSN: 16076729 NLM ISO Abbreviation: Dokl Biochem Biophys Subsets: MEDLINE
أسماء مطبوعة: Publication: New York, NY : Pleiades Publishing
Original Publication: Moscow, Russia : [Dordrecht] : International Academic Pub. Co. "Nauka/Interperiodica" ; Distributed worldwide by Kluwer Academic/Plenum Publishers, c2001-
مواضيع طبية MeSH: Disease Resistance* , Meristem*/genetics , Meristem*/metabolism , Meristem*/virology , Nuclear Proteins*/genetics , Nuclear Proteins*/metabolism , Osmotic Pressure* , Plant Proteins*/genetics , Plant Proteins*/metabolism , Solanum tuberosum*/genetics , Solanum tuberosum*/metabolism , Solanum tuberosum*/virology, Plant Diseases/*virology , Rhabdoviridae/*metabolism, CRISPR-Cas Systems
مستخلص: The role of the nuclear protein coilin in the mechanisms of resistance of potato Solanum tuberosum cultivar Chicago to biotic and abiotic stresses was studied using the CRISPR-Cas9 technology. For the coilin gene editing, a complex consisting of the Cas9 endonuclease and a short guide RNA was immobilized on gold or chitosan microparticles and delivered into apical meristem cells by bioballistics or vacuum infiltration methods, respectively. Editing at least one allele of the coilin gene considerably increased the resistance of the edited lines to infection with the potato virus Y and their tolerance to salt and osmotic stress.
References: Chromosoma. 2005 Aug;114(3):155-66. (PMID: 16003501)
Chromosoma. 2010 Apr;119(2):205-15. (PMID: 19997741)
Mol Cell. 2010 Oct 22;40(2):216-27. (PMID: 20965417)
PLoS One. 2013;8(1):e53571. (PMID: 23320094)
Nucleus. 2014 Jan-Feb;5(1):85-94. (PMID: 24637832)
Transgenic Res. 2014 Oct;23(5):743-56. (PMID: 25092532)
Plant Cell Rep. 2016 Jul;35(7):1469-74. (PMID: 27100964)
Plant Cell Rep. 2017 Jan;36(1):117-128. (PMID: 27699473)
RNA Biol. 2017 Jun 3;14(6):779-790. (PMID: 27726481)
Nat Commun. 2017 May 31;8:15464. (PMID: 28561021)
Dokl Biochem Biophys. 2018 Mar;479(1):90-94. (PMID: 29779105)
المشرفين على المادة: 0 (Nuclear Proteins)
0 (Plant Proteins)
136882-81-0 (p80-coilin)
SCR Organism: Potato yellow dwarf nucleorhabdovirus
تواريخ الأحداث: Date Created: 20190424 Date Completed: 20190502 Latest Revision: 20200225
رمز التحديث: 20221213
DOI: 10.1134/S1607672919010241
PMID: 31012023
قاعدة البيانات: MEDLINE
الوصف
تدمد:1608-3091
DOI:10.1134/S1607672919010241