دورية أكاديمية
Characterization of Anaplasma spp. infection in dogs from Costa Rica.
العنوان: | Characterization of Anaplasma spp. infection in dogs from Costa Rica. |
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المؤلفون: | Bonilla MC; Laboratorio de Entomología, Programa Investigación en Medicina Poblacional, Escuela de Medicina Veterinaria, Universidad Nacional, Campus Pbro. Benjamín Nuñez, Barreal Heredia, P.O. Box 86-3000, Heredia, Costa Rica., Campos-Calderón L; Laboratorio de Farmacología y Toxicología, Escuela de Medicina Veterinaria, Universidad Nacional, Campus Pbro. Benjamín Nuñez, Barreal Heredia, P.O. Box 86-3000, Heredia, Costa Rica., Jiménez-Rocha AE; Laboratorio de Parasitología, Escuela de Medicina Veterinaria, Universidad Nacional, Campus Pbro. Benjamín Nuñez, Barreal Heredia, P.O. Box 86-3000, Heredia, Costa Rica., Romero-Zúñiga JJ; Laboratorio de Entomología, Programa Investigación en Medicina Poblacional, Escuela de Medicina Veterinaria, Universidad Nacional, Campus Pbro. Benjamín Nuñez, Barreal Heredia, P.O. Box 86-3000, Heredia, Costa Rica., Alberti A; Departmento de Medicina Veterinaria, Universidad de Sassari, Italy., Zobba R; Departmento de Medicina Veterinaria, Universidad de Sassari, Italy., Dolz G; Laboratorio de Entomología, Programa Investigación en Medicina Poblacional, Escuela de Medicina Veterinaria, Universidad Nacional, Campus Pbro. Benjamín Nuñez, Barreal Heredia, P.O. Box 86-3000, Heredia, Costa Rica. Electronic address: gaby.dolz.wiedner@una.cr. |
المصدر: | Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2017 May; Vol. 8, pp. 60-65. Date of Electronic Publication: 2017 Feb 09. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101680410 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-9390 (Electronic) Linking ISSN: 24059390 NLM ISO Abbreviation: Vet Parasitol Reg Stud Reports Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier, [2015]- |
مستخلص: | A cross-sectional study combining serological and molecular techniques for detecting selected Anaplasma species was conducted between 2011 and 2012 in dogs and ticks sampled in all provinces of Costa Rica. Global Anaplasma spp. seroprevalence was 2.7% (11/408) by indirect immunofluorescence assay. The 16S rRNA PCR confirmed active A. phagocytophilum infection only in one dog (0.3%, 1/374); however, the same sample was negative to groEL PCR. Out of 122 Rhipicephalus sanguineus s.l. ticks analyzed, one (0.8%) was found positive to A. phagocytophilum 16S rRNA PCR but negative when tested by groEL PCR; this tick was collected from a seronegative and PCR negative dog. Both 16S rRNA sequences were 100% (510/510bp) identical to A. phagocytophilum strains isolated in different countries from different hosts. The presence of A. platys was established in four dogs (1%, 4/374) by both 16SrRNA and groEL PCR. Ticks collected from the same dogs tested negative by PCR. The 16S rRNA sequences were 100% identical to the corresponding sequences of A. platys strains isolated from dogs in Croatia and Brazil, however groEL sequences showed variable similarity levels (99-100%) with different strains of A. platys isolated in Chile, Japan and Thailand, pointing out the possible presence of different variants in Central America. Collectively data indicate low prevalence of A. phagocytophilum and A. platys in dogs from Costa Rica. Furthermore, infections seem to occur without clinical signs but with some hematological changes, and seem to resolve without treatment. (Copyright © 2017 Elsevier B.V. All rights reserved.) |
فهرسة مساهمة: | Keywords: Anaplasma phagocytophilum; Anaplasma platys; PCR; Serology |
تواريخ الأحداث: | Date Created: 20190425 Latest Revision: 20191120 |
رمز التحديث: | 20240628 |
DOI: | 10.1016/j.vprsr.2017.02.003 |
PMID: | 31014639 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2405-9390 |
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DOI: | 10.1016/j.vprsr.2017.02.003 |