دورية أكاديمية
Berberine hydrochloride protects against cytokine-induced inflammation through multiple pathways in undifferentiated C2C12 myoblast cells.
| العنوان: | Berberine hydrochloride protects against cytokine-induced inflammation through multiple pathways in undifferentiated C2C12 myoblast cells. |
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| المؤلفون: | Poudel A; a Physician Assistant Program, College of Health Professions, Central Michigan University, Mount Pleasant, MI 48859, USA., Zhou JY; b College of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA., Mekala N; b College of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA., Welchko R; a Physician Assistant Program, College of Health Professions, Central Michigan University, Mount Pleasant, MI 48859, USA., Rosca MG; b College of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA., Li L; a Physician Assistant Program, College of Health Professions, Central Michigan University, Mount Pleasant, MI 48859, USA. |
| المصدر: | Canadian journal of physiology and pharmacology [Can J Physiol Pharmacol] 2019 Aug; Vol. 97 (8), pp. 699-707. Date of Electronic Publication: 2019 Apr 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372712 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1205-7541 (Electronic) Linking ISSN: 00084212 NLM ISO Abbreviation: Can J Physiol Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : Ottawa, ON : Canadian Science Publishing Original Publication: Ottawa, National Research Council of Canada. |
| مواضيع طبية MeSH: | Anti-Inflammatory Agents/*pharmacology , Berberine/*pharmacology , Cytokines/*pharmacology , Myoblasts/*drug effects , Myoblasts/*pathology, Active Transport, Cell Nucleus/drug effects ; Animals ; Antioxidants/metabolism ; Cell Nucleus/drug effects ; Cell Nucleus/metabolism ; Cytoprotection/drug effects ; Inflammation/chemically induced ; Inflammation/metabolism ; Inflammation/pathology ; JNK Mitogen-Activated Protein Kinases/metabolism ; Mice ; Mitogen-Activated Protein Kinases/metabolism ; Myoblasts/metabolism ; NF-kappa B/metabolism ; Oxidative Stress/drug effects ; Signal Transduction/drug effects ; Tumor Necrosis Factor-alpha/metabolism ; p38 Mitogen-Activated Protein Kinases/metabolism |
| مستخلص: | Obesity is associated with skeletal muscle insulin resistance and the development of metabolic syndrome. Undifferentiated skeletal muscle cells are sensitive to oxidative stress. Berberine hydrochloride (BBR) improves insulin resistance and exhibits anti-inflammatory properties. However, the underlying mechanism and the cell signaling pathways involved remain largely elusive. We therefore investigated the anti-inflammatory effects of BBR and the signaling pathways using skeletal C2C12 myoblast cells. Undifferentiated C2C12 myoblast cells were treated with interleukin-1β alone or in combination with tumor necrosis factor-α in the presence or absence of BBR. We found that BBR reduced the cytokine-induced expression of inducible nitric oxide synthase and stress-related kinases including p-38 mitogen-activated protein kinase, nuclear factor kappa B (NF-κB), and stress-activated protein kinases/Jun amino-terminal kinases (SAPK/JNK) in C2C12 myoblast cells. Furthermore, BBR reversed cytokine-mediated suppression of AMP-activated protein kinase (AMPKα), sirtuin-1 (SIRT-1), and PPAR-γ coactivator-1α (PGC-1α). In addition, cytokine-induced reduction of mitochondrial marker proteins and function were rescued after BBR treatment. Catalase, an antioxidant enzyme, was elevated after BBR treatment. Our results demonstrate that BBR ameliorates cytokine-induced inflammation. The anti-inflammatory effect of BBR in skeletal progenitor cells is mediated through pathways including activation of the AMPKα-SIRT-1-PGC-1α, inhibition of the mitogen-activated protein kinase 4 (MKK4)-SAPK/JNK-C-JUN, as well as protection of mitochondrial bioenergetics. BBR may be a potential medication for metabolic syndrome. |
| فهرسة مساهمة: | Keywords: berberine; berbérine; cytokine; inflammation; insulin resistance; résistance à l’insuline |
| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Antioxidants) 0 (Cytokines) 0 (NF-kappa B) 0 (Tumor Necrosis Factor-alpha) 0I8Y3P32UF (Berberine) EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases) EC 2.7.11.24 (Mitogen-Activated Protein Kinases) EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20190427 Date Completed: 20191218 Latest Revision: 20191218 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1139/cjpp-2018-0653 |
| PMID: | 31026403 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1205-7541 |
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| DOI: | 10.1139/cjpp-2018-0653 |