دورية أكاديمية
Serologic methods for detection of Pasteurella multocida infections in nasal culture negative rabbits.
| العنوان: | Serologic methods for detection of Pasteurella multocida infections in nasal culture negative rabbits. |
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| المؤلفون: | Holmes HT, Matsumoto M, Patton NM, Zehfus BR |
| المصدر: | Laboratory animal science [Lab Anim Sci] 1986 Dec; Vol. 36 (6), pp. 640-5. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for Laboratory Animal Science Country of Publication: United States NLM ID: 1266503 Publication Model: Print Cited Medium: Print ISSN: 0023-6764 (Print) Linking ISSN: 00236764 NLM ISO Abbreviation: Lab Anim Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Memphis, TN : American Association for Laboratory Animal Science Original Publication: Joliet, Ill., American Assn. for Laboratory Animal Science. |
| مواضيع طبية MeSH: | Pasteurella Infections/*diagnosis, Animals ; Animals, Laboratory ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; Immunodiffusion/methods ; Male ; Nasal Mucosa/microbiology ; Pasteurella/isolation & purification ; Rabbits ; Serotyping |
| مستخلص: | An agar gel-diffusion test (AGDT) and an enzyme-linked immunosorbent assay (ELISA) were utilized to detect serum antibodies against Pasteurella multocida in naturally infected rabbits derived originally from a Pasteurella-free colony. The antigen used in both assays was purified from a serotype 3 (P-1059) strain of P. multocida. Among 47 serum samples tested 15 (32%) were seropositive; 12 (26%) of which were both AGDT and ELISA-positive, while 3 (6%) were ELISA-positive only. All rabbits examined were normal clinically and negative to repeated nasal cultures, but subsequent cultures at necropsy demonstrated the presence of P. multocida in 11 of the AGDT-positive rabbits and in 14 of the ELISA-positive rabbits. The organism was isolated most frequently from the naso-oropharynx and the tympanic bullae. Serotyping of isolates recovered from the nasopharynx were determined to be serotype 3 or 3,12. Ten seronegative rabbits also were necropsied and none were found harboring P. multocida. These preliminary data indicate that the application of an enzyme-linked immunosorbent assay may prove efficacious in identifying apparently healthy, consistently nasal culture-negative rabbits as subclinical carriers of P. multocida. |
| تواريخ الأحداث: | Date Created: 19861201 Date Completed: 19870408 Latest Revision: 20031114 |
| رمز التحديث: | 20221213 |
| PMID: | 3102851 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0023-6764 |
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