دورية أكاديمية
أعرض في EDS

Isolation of Antimicrobial Compounds From Cnestis ferruginea Vahl ex. DC (Connaraceae) Leaves Through Bioassay-Guided Fractionation.

التفاصيل البيبلوغرافية
العنوان: Isolation of Antimicrobial Compounds From Cnestis ferruginea Vahl ex. DC (Connaraceae) Leaves Through Bioassay-Guided Fractionation.
المؤلفون: Kouakou K; UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire., Panda SK; Department of Biology, Katholieke Universiteit Leuven, Leuven, Belgium., Yang MR; State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China., Lu JG; State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China., Jiang ZH; State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China., Van Puyvelde L; Department of Biology, Katholieke Universiteit Leuven, Leuven, Belgium., Luyten W; Department of Biology, Katholieke Universiteit Leuven, Leuven, Belgium.
المصدر: Frontiers in microbiology [Front Microbiol] 2019 Apr 11; Vol. 10, pp. 705. Date of Electronic Publication: 2019 Apr 11 (Print Publication: 2019).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Different parts of Cnestis ferruginea are used in traditional African medicine for treating infectious diseases such as dysentery, bronchitis, eye troubles, conjunctivitis, sinusitis, gonorrhea, and syphilis. Despite its long traditional use in the treatment of infections, this plant is not well studied for its in vitro antimicrobial properties. Therefore, the present study aims to establish the antimicrobial activity profile of extracts from this plant, as well as to isolate and evaluate the antimicrobial activity of the most abundant bioactive compound in C. ferruginea leaves through bioassay-guided purification, using Staphylococcus aureus as a target organism. Although both methanol and water extracts of the plant leaves proved active against S. aureus , a water extract was pursued, and subjected further to liquid-liquid partitioning (ethyl acetate, butanol, and water). The ethyl acetate fraction was found to be the most potent and was subjected to silica gel chromatography. In total, 250 fractions were obtained, and those with similar TLC profiles were clustered into 22 major groups, of which pooled fraction-F6 (83 mg) was the most potent. Additional purification by HPLC resulted in two active peaks, which were identified, using a combination of NMR and mass spectrometry, as hydroquinone and caffeic acid methyl ester. Their antimicrobial activity was confirmed using a microdilution protocol on S. aureus , where hydroquinone had a stronger activity (MIC 50 = 63 μg/mL) compared to caffeic acid methyl ester (>200 μg/mL). Traditionally this plant is used as an aqueous preparation to treat many infections, and the present study also demonstrated antimicrobial activity in the aqueous extract, which appears due mainly to two major water-soluble compounds isolated through bioassay-guided purification. This supports the clinical use of the aqueous extract of C. ferruginea leaves as a phytotherapeutic for bacterial infections.
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فهرسة مساهمة: Keywords: Cnestis ferruginea; S. aureus; benzene-1,4-diol; bioassay-guided fractionation; caffeic acid methyl ester; traditional african medicine
تواريخ الأحداث: Date Created: 20190430 Latest Revision: 20231011
رمز التحديث: 20240513
مُعرف محوري في PubMed: PMC6470257
DOI: 10.3389/fmicb.2019.00705
PMID: 31031719
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2019.00705