دورية أكاديمية
Use of a fluorinated probe to quantitatively monitor amino acid binding preferences of ruthenium(ii) arene complexes.
| العنوان: | Use of a fluorinated probe to quantitatively monitor amino acid binding preferences of ruthenium(ii) arene complexes. |
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| المؤلفون: | Biggs GS; University of Cambridge, Chemistry Department, Lensfield Road, Cambridge CB2 1EW, UK. pdb30@cam.ac.uk srb39@cam.ac.uk., O'Neill MJ, Carames Mendez P, Scrase TG, Lin Y, Bin-Maarof AM, Bond AD, Boss SR, Barker PD |
| المصدر: | Dalton transactions (Cambridge, England : 2003) [Dalton Trans] 2019 May 21; Vol. 48 (20), pp. 6910-6920. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101176026 Publication Model: Print Cited Medium: Internet ISSN: 1477-9234 (Electronic) Linking ISSN: 14779226 NLM ISO Abbreviation: Dalton Trans Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : Royal Society of Chemistry, c2003- |
| مواضيع طبية MeSH: | Amino Acids/*analysis , Coordination Complexes/*chemistry , Fluorine/*chemistry , Ruthenium/*chemistry, Coordination Complexes/chemical synthesis ; Cysteine/chemistry ; Halogenation ; Kinetics ; Ligands ; Molecular Structure ; Water/chemistry |
| مستخلص: | In order to address outstanding questions about ruthenium complexes in complex biological solutions, 19F NMR spectroscopy was used to follow the binding preferences between fluorinated RuII(η6-arene)(bipyridine) complexes and protected amino acids and glutathione. Reporting what ruthenium compounds bind to in complex environments has so far been restricted to relatively qualitative methods, such as mass spectrometry and X-ray spectroscopic methods; however, quantitative information on the species present in the solution phase cannot be inferred from these techniques. Furthermore, using 1H NMR, in water, to distinguish and monitor a number of different complex RuII(η6-arene) adducts forming is challenging. Incorporating an NMR active heteroatom into ruthenium organometallic complexes provides a quantitative, diagnostic 'fingerprint' to track solution-phase behaviour and allow for unambiguous assignment of any given adduct. The resulting 19F NMR spectra show for the first time the varied, dynamic behaviour of organoruthenium compounds when exposed to simple biomolecules in complex mixtures. The rates of formation of the different observed species are dramatically influenced by the electronic properties at the metal, even in a closely related series of complexes in which only the electron-donating properties of the arene ligand are altered. Preference for cysteine binding is absolute: the first quantitative solution-phase evidence of such behaviour. |
| المشرفين على المادة: | 0 (Amino Acids) 0 (Coordination Complexes) 0 (Ligands) 059QF0KO0R (Water) 284SYP0193 (Fluorine) 7UI0TKC3U5 (Ruthenium) K848JZ4886 (Cysteine) |
| تواريخ الأحداث: | Date Created: 20190501 Date Completed: 20190603 Latest Revision: 20190603 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1039/c8dt05159c |
| PMID: | 31038129 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9234 |
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| DOI: | 10.1039/c8dt05159c |