دورية أكاديمية
Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells.
| العنوان: | Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells. |
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| المؤلفون: | Simonović J, Toljić B, Rašković B, Jovanović V, Lazarević M, Milošević M, Nikolić N, Panajotović R, Milašin J; Jelena Milašin, School of Dental Medicine, University of Belgrade, Dr Subotića 8, 11000 Belgrade, Serbia, jelena.milasin@stomf.bg.ac.rs. |
| المصدر: | Croatian medical journal [Croat Med J] 2019 Apr 30; Vol. 60 (2), pp. 78-86. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Medicinska Naklada Country of Publication: Croatia NLM ID: 9424324 Publication Model: Print Cited Medium: Internet ISSN: 1332-8166 (Electronic) Linking ISSN: 03539504 NLM ISO Abbreviation: Croat Med J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2001- : Zagreb : Medicinska Naklada Original Publication: Zagreb : University of Zagreb Medical School, [1992- |
| مواضيع طبية MeSH: | Spectrum Analysis, Raman*, Dental Pulp/*cytology , Dental Sac/*cytology , Mesenchymal Stem Cells/*chemistry , Molar, Third/*cytology, Adolescent ; Cell Differentiation ; Flow Cytometry ; Humans ; Real-Time Polymerase Chain Reaction ; Stem Cells ; Tooth |
| مستخلص: | Aim: To characterize stem cells originating from different dental tissues (apical papilla [SCAP], dental follicle [DFSC], and pulp [DPSC]) and test the capacity of Raman microspectroscopy to distinguish between the three dental stem cell types. Methods: SCAP, DFSC, and DPSC cultures were generated from three immature wisdom teeth originating from three patients. Cell stemness was confirmed by inducing neuro-, osteo-, chondro-, and adipo-differentiaton and by mesenchymal marker expression analysis by flow-cytometry and real-time polymerase chain reaction. Cellular components were then evaluated by Raman microspectroscopy. Results: We found differences between SCAP, DFSC, and DPSC Raman spectra. The ratio between proteins and nucleic acids (748/770), a parameter for discriminating more differentiated from less differentiated cells, showed significant differences between the three cell types. All cells also displayed a fingerprint region in the 600-700 cm-1 range, and characteristic lipid peaks at positions 1440 cm-1 and 1650 cm-1. Conclusion: Although different dental stem cells exhibited similar Raman spectra, the method enabled us to make subtle distinction between them. |
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| تواريخ الأحداث: | Date Created: 20190503 Date Completed: 20190705 Latest Revision: 20200225 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC6509629 |
| PMID: | 31044579 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1332-8166 |
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