دورية أكاديمية
Circulation of imipenem-resistant Acinetobacter baumannii ST10, ST2 and ST3 in a university teaching hospital from Tehran, Iran.
| العنوان: | Circulation of imipenem-resistant Acinetobacter baumannii ST10, ST2 and ST3 in a university teaching hospital from Tehran, Iran. |
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| المؤلفون: | Abhari SS; 1 Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Gilan, Iran., Badmasti F; 2 Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Modiri L; 1 Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Gilan, Iran., Aslani MM; 2 Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran., Asmar M; 3 Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran. |
| المصدر: | Journal of medical microbiology [J Med Microbiol] 2019 Jun; Vol. 68 (6), pp. 860-865. Date of Electronic Publication: 2019 May 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0224131 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-5644 (Electronic) Linking ISSN: 00222615 NLM ISO Abbreviation: J Med Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: 1968-1995: Edinburgh, Livingstone. |
| مواضيع طبية MeSH: | Acinetobacter Infections/*epidemiology , Acinetobacter baumannii/*genetics , Anti-Bacterial Agents/*pharmacology , Drug Resistance, Multiple, Bacterial/*genetics , Imipenem/*pharmacology, Acinetobacter Infections/microbiology ; Acinetobacter baumannii/drug effects ; Acinetobacter baumannii/enzymology ; Adult ; Aged ; Female ; Hospitals, Teaching ; Hospitals, University ; Humans ; Iran/epidemiology ; Male ; Middle Aged ; Multilocus Sequence Typing |
| مستخلص: | Purpose: Multi-drug resistant (MDR) Acinetobacter baumannii has introduced a worldwide health crisis. The purposes of this study were to characterize the clonal relatedness among MDR clinical strains and to introduce a new two-locus typing method confirmed by multi-locus sequence typing (MLST). Methodology: In this study, we determined antimicrobial resistance, detected genes associated with carbapenem resistance and characterized clonal relatedness among 99 clinical isolates extracted from 82 hospitalized inpatients in a university hospital. Results: Of the 99 A. baumannii isolates, 92.9% (92/99) were resistant to imipenem and 97.9% (97/99) had an MDR profile. We found that the high prevalence of blaVIM [94.9% (94/99)] and blaOXA-23-like [93.93% (93/99)] is the main mechanism of carbapenem resistance. This study proposes a new two-locus typing (blaOXA-51-like and ampC) method for the rapid identification of clonal complexes (CCs). The results of this method and confirmation by MLST show that clinical isolates carry blaOXA-68 as well as ampC-10 or ampC-20 genes belonging to CC10 (ST10); blaOXA-66 and ampC-2 belonging to CC2 (ST2); and blaOXA-71 and ampC-3 belonging to CC3 (ST3). One isolate had blaOXA-90 with an undetermined allele number of ampC belonging to ST513. Conclusion: The high prevalence of MDR strains and the circulation of four limited clones, including ST10 (45/99), ST2 (41/99), ST3 (12/99) and ST513 (1/99), in the clinical setting highlights the importance of a rigorous infection control programme. The two-locus typing method has more discrimination than the application of each method separately and it could be applied for the rapid determination of the CC without performing MLST. |
| فهرسة مساهمة: | Keywords: Multi-drug resistant (MDR) Acinetobacter baumannii; carbapenemase genes; clonal relatedness |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 71OTZ9ZE0A (Imipenem) |
| تواريخ الأحداث: | Date Created: 20190504 Date Completed: 20190610 Latest Revision: 20190613 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1099/jmm.0.000987 |
| PMID: | 31050632 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1473-5644 |
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| DOI: | 10.1099/jmm.0.000987 |