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Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil.

التفاصيل البيبلوغرافية
العنوان: Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil.
المؤلفون: Mendes NS; Agricultural and Livestock Microbiology Graduation Program, School of Agricultural and Veterinarian Sciences, São Paulo State University (UNESP), Jaboticabal, Brazil.; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, São Paulo, 14884-900, Brazil., de Souza Ramos IA; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, São Paulo, 14884-900, Brazil., Herrera HM; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., Campos JBV; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., de Almeida Alves JV; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., de Macedo GC; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., Machado RZ; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, São Paulo, 14884-900, Brazil., André MR; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, Jaboticabal, São Paulo, 14884-900, Brazil. mr.andre@unesp.br.
المصدر: Parasitology research [Parasitol Res] 2019 Jul; Vol. 118 (7), pp. 2027-2040. Date of Electronic Publication: 2019 May 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: Germany NLM ID: 8703571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1955 (Electronic) Linking ISSN: 09320113 NLM ISO Abbreviation: Parasitol Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Springer International, c1987-
مواضيع طبية MeSH: Genetic Variation*, Antigens, Protozoan/*genetics , Babesia bovis/*genetics , Babesiosis/*parasitology , Cattle Diseases/*parasitology, Amino Acid Sequence ; Animals ; Antigens, Surface/genetics ; Babesiosis/epidemiology ; Brazil ; Cattle ; Cattle Diseases/epidemiology ; Entropy ; Merozoites ; Phylogeny ; Polymerase Chain Reaction/veterinary ; Wetlands
مستخلص: Babesia bovis is the etiological agent of bovine babesiosis, a disease transmitted by Rhipicephalus microplus, which affects cattle herds in tropical and subtropical regions of the world, causing significant economic losses due to decreasing meat and milk yield. This study used molecular techniques to determine the occurrence and genetic diversity of B. bovis, based on the genes encoding the spherical body protein (sbp-2) and the merozoite surface antigens (MSAs) genes, in a herd of 400 Nellore (Bos indicus) sampled from beef cattle farms in the Pantanal region, state of Mato Grosso do Sul, Midwestern Brazil. The results of the nested PCR assays based on the sbp-2 gene indicated that 18 (4.5%) calves were positive for B. bovis; out of them, while 77.7% (14/18) were positive for the B. bovis msa-2b fragment, 66.6% (12/18) were positive for the msa-2c fragment. The phylogenetic analysis based on the maximum likelihood method using 14 sequences from msa-2b clones and 13 sequences from msa-2c clones indicated that the sequences detected in this study are clearly distributed in different cladograms. These findings corroborated the diversity analysis of the same sequences, which revealed the presence of 14 and 11 haplotypes of the msa-2b and msa-2c genes, respectively. Furthermore, the entropy analyses of amino acid sequences revealed 78 and 44 high entropy peaks with values ranging from 0.25 to 1.53 and from 0.27 to 1.09 for MSA-2B and MSA-2C, respectively. Therefore, the results indicate a low molecular occurrence of B. bovis in beef cattle sampled in the Brazilian Pantanal. Despite this, a high degree of genetic diversity was found in the analyzed B. bovis population, with possibly different haplotypes coexisting in the same animal and/or in the same studied herd.
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فهرسة مساهمة: Keywords: Babesia bovis; Bovine babesiosis; Genetic diversity; MSA; Pantanal
المشرفين على المادة: 0 (Antigens, Protozoan)
0 (Antigens, Surface)
تواريخ الأحداث: Date Created: 20190513 Date Completed: 20191009 Latest Revision: 20200225
رمز التحديث: 20231215
DOI: 10.1007/s00436-019-06337-3
PMID: 31079252
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-1955
DOI:10.1007/s00436-019-06337-3