دورية أكاديمية
Fungal Exocellular (1-6)-β-d-glucan: Carboxymethylation, Characterization, and Antioxidant Activity.
| العنوان: | Fungal Exocellular (1-6)-β-d-glucan: Carboxymethylation, Characterization, and Antioxidant Activity. |
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| المؤلفون: | Theis TV; Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Via do Conhecimento, km 01, 85503-390 Pato Branco, Paraná, Brazil. thaisvtheis@gmail.com., Queiroz Santos VA; Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Via do Conhecimento, km 01, 85503-390 Pato Branco, Paraná, Brazil. vidianyqueiroz@yahoo.com.br., Appelt P; Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Rua Rosalina Maria Ferreira, 1233, 87301-899 Campo Mourão, Paraná, Brazil. patriciaappelt18@gmail.com., M Barbosa-Dekker A; Centro de Ciências Exatas, Departamento de Química, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, 86057-970 Londrina, Paraná, Brazil. anelibarbosa@gmail.com., Vetvicka V; Department of Pathology, University of Louisville, 511 S. Floyd St, Louisville, KY 40292, USA. Vaclav.vetvicka@louisville.edu., F H Dekker R; Programa de Pós-Graduação em Engenharia Ambiental, Universidade Tecnológica Federal do Paraná (UTFPR), Avenida dos Pioneiros 3131, 86036-370 Londrina, Paraná, Brazil. xylanase@gmail.com., A Cunha MA; Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Via do Conhecimento, km 01, 85503-390 Pato Branco, Paraná, Brazil. mcunha@utfpr.edu.br. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2019 May 11; Vol. 20 (9). Date of Electronic Publication: 2019 May 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Antioxidants/*pharmacology , Ascomycota/*chemistry , beta-Glucans/*metabolism , beta-Glucans/*pharmacology, Free Radical Scavengers/chemistry ; Methylation ; Spectroscopy, Fourier Transform Infrared ; Temperature ; Thermogravimetry ; X-Ray Diffraction ; beta-Glucans/chemistry |
| مستخلص: | Exocellular (1→6)-β-d-glucan (lasiodiplodan) produced by the fungus Lasiodiplodia theobromae MMPI was derivatized by carboxymethylation using different concentrations of a derivatizing agent. Lasiodiplodan was derivatized by carboxymethylation in an attempt to increase its solubility and enhance its biological activities. Carboxymethylglucans with degrees of substitution (DS) of 0.32, 0.47, 0.51, 0.58, and 0.68 were produced and characterized. FTIR analysis showed a band of strong intensity at 1600 cm -1 and an absorption band at 1421 cm -1 , resulting from asymmetric and symmetrical stretching vibrations, respectively, of the carboxymethyl group COO- in the carboxymethylated samples. Thermal analysis showed that native lasiodiplodan (LN) and carboxymethylated derivatives (LC) exhibited thermal stability up to 200-210 °C. X-ray diffractometry demonstrated that both native and carboxymethylated lasiodiplodan presented predominantly an amorphous nature. Scanning electron microscopy revealed that carboxymethylation promoted morphological changes in the biopolymer and increased porosity, and alveolar structures were observed along the surface. The introduction of carboxymethyl groups in the macromolecule promoted increased solubility and potentiated the hydroxyl radical-scavenging activity, suggesting a correlation between degree of substitution and antioxidant activity. |
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| فهرسة مساهمة: | Keywords: bioactive macromolecules; biopolymer; carbohydrate; exopolysaccharide; lasiodiplodan |
| المشرفين على المادة: | 0 (Antioxidants) 0 (Free Radical Scavengers) 0 (beta-Glucans) |
| تواريخ الأحداث: | Date Created: 20190515 Date Completed: 20190903 Latest Revision: 20200225 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC6539860 |
| DOI: | 10.3390/ijms20092337 |
| PMID: | 31083511 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms20092337 |