Impact of Distinct Freezing Curves on the Quality of Ram Sperm after Thawing.

التفاصيل البيبلوغرافية
العنوان:	Impact of Distinct Freezing Curves on the Quality of Ram Sperm after Thawing.
المؤلفون:	Ferreira CER; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Goularte KL; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., da Cruz Tavares G; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Gheller SMM; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Mondadori RG; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Instituto de Biologia,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Vieira AD; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Lucia T Jr; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil. thomaz@ufpel.edu.br, thomaz@pq.cnpq.br.
المصدر:	Cryo letters [Cryo Letters] 2019 May/Jun; Vol. 40 (3), pp. 193-199.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: CryoLetters Country of Publication: England NLM ID: 9891832 Publication Model: Print Cited Medium: Print ISSN: 0143-2044 (Print) Linking ISSN: 01432044 NLM ISO Abbreviation: Cryo Letters
أسماء مطبوعة:	Publication: London, Eng. : CryoLetters Original Publication: [London : Royal Veterinary College,
مواضيع طبية MeSH:	Sperm Motility, Cryopreservation/veterinary , Semen Preservation/veterinary , Spermatozoa/physiology, Animals ; Freezing ; Male ; Sheep
مستخلص:	Background: Automated equipment with customized freezing curves can be used to cryopreserve ram sperm, but none is considered a standard. Objective: This study compared the post-thawing quality of ram sperm frozen using a conventional freezing curve and two controlled-rate curves. Materials and Methods: Six ejaculates were collected from four rams (n = 24). In the conventional curve (110 min), sperm was cooled at -0.3 to -0.5°C min ^-1 until 5°C, stabilized for 60 min and exposed to liquid nitrogen (LN 2 ) vapor for 10 min (-80°C) before submersion. The slow-customized (SC) curve (126.2 min) used a rate of -0.25°C min ^-1 until 5°C, stabilization for 60 min and a rate of -20°C min ^-1 until -120°C before immersion in LN 2 . Rates for the fast-customized (FC) curve (75 min) were: -0.3°C min ^-1 until 5°C; -3°C min ^-1 until -10°C; -5°C min ^-1 until -35°C; and -4°C min ^-1 until immersion in LN 2 (-43°C). Results: Velocity in a straight line and beat-cross frequency were lower for spermatozoa frozen with the FC than with the conventional curve (P < 0.05). The FC curve resulted in more membrane and acrosome damages than the other curves (P < 0.05). Mitochondrial membrane potential was lower with the SC than with the other curves (P < 0.05). Conclusion: The conventional curve was more efficient than both tested automated freezing curves. The FC curve may be an alternative to the SC curve due to the shorter processing time.
تواريخ الأحداث:	Date Created: 20190517 Date Completed: 20190802 Latest Revision: 20190802
رمز التحديث:	20221213
PMID:	31095669
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	0143-2044