دورية أكاديمية
Impact of Distinct Freezing Curves on the Quality of Ram Sperm after Thawing.
العنوان: | Impact of Distinct Freezing Curves on the Quality of Ram Sperm after Thawing. |
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المؤلفون: | Ferreira CER; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Goularte KL; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., da Cruz Tavares G; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Gheller SMM; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Mondadori RG; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Instituto de Biologia,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Vieira AD; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil., Lucia T Jr; ReproPel, Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil; Faculdade de Veterinária,Universidade Federal de Pelotas, 96010-900, Pelotas-RS, Brazil. thomaz@ufpel.edu.br, thomaz@pq.cnpq.br. |
المصدر: | Cryo letters [Cryo Letters] 2019 May/Jun; Vol. 40 (3), pp. 193-199. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: CryoLetters Country of Publication: England NLM ID: 9891832 Publication Model: Print Cited Medium: Print ISSN: 0143-2044 (Print) Linking ISSN: 01432044 NLM ISO Abbreviation: Cryo Letters |
أسماء مطبوعة: | Publication: London, Eng. : CryoLetters Original Publication: [London : Royal Veterinary College, |
مواضيع طبية MeSH: | Sperm Motility*, Cryopreservation/*veterinary , Semen Preservation/*veterinary , Spermatozoa/*physiology, Animals ; Freezing ; Male ; Sheep |
مستخلص: | Background: Automated equipment with customized freezing curves can be used to cryopreserve ram sperm, but none is considered a standard. Objective: This study compared the post-thawing quality of ram sperm frozen using a conventional freezing curve and two controlled-rate curves. Materials and Methods: Six ejaculates were collected from four rams (n = 24). In the conventional curve (110 min), sperm was cooled at -0.3 to -0.5°C min -1 until 5°C, stabilized for 60 min and exposed to liquid nitrogen (LN Results: Velocity in a straight line and beat-cross frequency were lower for spermatozoa frozen with the FC than with the conventional curve (P < 0.05). The FC curve resulted in more membrane and acrosome damages than the other curves (P < 0.05). Mitochondrial membrane potential was lower with the SC than with the other curves (P < 0.05). Conclusion: The conventional curve was more efficient than both tested automated freezing curves. The FC curve may be an alternative to the SC curve due to the shorter processing time. |
تواريخ الأحداث: | Date Created: 20190517 Date Completed: 20190802 Latest Revision: 20190802 |
رمز التحديث: | 20221213 |
PMID: | 31095669 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0143-2044 |
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