دورية أكاديمية
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An isolate of sweet potato chlorotic stunt virus from Brazil with a distinct genome organization.

التفاصيل البيبلوغرافية
العنوان: An isolate of sweet potato chlorotic stunt virus from Brazil with a distinct genome organization.
المؤلفون: Rossato M; Departamento de Fitopatologia, Universidade de Brasília, Brasília, Brazil., Souza CA; Departamento de Fitopatologia, Universidade de Brasília, Brasília, Brazil., Melo FL; Departamento de Fitopatologia, Universidade de Brasília, Brasília, Brazil., Orilio AF; Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil.; Departamento de Fitopatologia, Universidade Federal de Viçosa, Minas Gerais, Brazil., Resende RO; Departamento de Fitopatologia, Universidade de Brasília, Brasília, Brazil.; Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil., Ribeiro SG; Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil., Naito FYB; Departamento de Fitopatologia, Universidade de Brasília, Brasília, Brazil., Ribeiro GP; Departamento de Fitopatologia, Universidade Federal Rural de Pernambuco, Pernambuco, Brazil., Andrade GP; Departamento de Fitopatologia, Universidade Federal Rural de Pernambuco, Pernambuco, Brazil., Pereira-Carvalho RC; Departamento de Fitopatologia, Universidade de Brasília, Brasília, Brazil. rcpcarvalho@unb.br.
المصدر: Archives of virology [Arch Virol] 2019 Aug; Vol. 164 (8), pp. 2175-2178. Date of Electronic Publication: 2019 May 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7506870 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-8798 (Electronic) Linking ISSN: 03048608 NLM ISO Abbreviation: Arch Virol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Wien, New York, Springer-Verlag.
مواضيع طبية MeSH: Crinivirus/*genetics , Crinivirus/*isolation & purification , Genome, Viral/*genetics, Brazil ; Crinivirus/classification ; Ipomoea batatas/virology ; Open Reading Frames/genetics ; Phylogeny ; Plant Diseases/virology ; RNA, Viral/genetics ; Viral Proteins/genetics
مستخلص: Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae), is an economically important pathogen of sweet potato. In the present work, the nucleotide sequences of two RNA segments of SPCSV (isolate SPCSV-UNB-01) were determined by MiSeq Illumina sequencing of samples of sweet potato plants grafted onto Ipomoea setosa. A comparative analysis of the genome organization of SPCSV-UNB-01 and other SPCSV sequences showed that RNA1 was lacking p22, and p5.1 and that p5.2. was absent in RNA2, indicating a unique genomic pattern. SPCSV-UNB-01 contained longer p6 and p5 regions, with little similarity to orthologous sequences. Sequence comparison did not reveal any previously identified functional domains within these open reading frames (ORFs). No recombination or rearrangement events were detected. Phylogenetic analysis suggested the possibility of separate entries of SPCSV into South America based on the genetic distance between SPCSV-UNB-01 and the Peruvian isolate m2-47. Samples from northeastern Brazil (State of Pernambuco) were positive for SPCSV when tested using specific primers for the major coat protein (CP) gene. This is the first full-length genome sequence of SPCSV-UNB-01 from Brazil.
المشرفين على المادة: 0 (RNA, Viral)
0 (Viral Proteins)
SCR Organism: Sweet potato chlorotic stunt virus
تواريخ الأحداث: Date Created: 20190519 Date Completed: 20190703 Latest Revision: 20190703
رمز التحديث: 20240628
DOI: 10.1007/s00705-019-04281-2
PMID: 31102051
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-8798
DOI:10.1007/s00705-019-04281-2