دورية أكاديمية
Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model.
| العنوان: | Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model. |
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| المؤلفون: | Laulund ASB; Department of Plastic Surgery, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark., Trøstrup H; Department of Plastic Surgery, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark., Lerche CJ; Department of Hematology, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, 2100 Copenhagen, Denmark., Thomsen K; Department of Plastic Surgery, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark., Christophersen L; Department of Plastic Surgery, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark., Calum H; Department of Clinical Microbiology, Amager and Hvidovre Hospital, Copenhagen University Hospital, Kettegaard Alle 30, 2650 Copenhagen, Denmark., Høiby N; Institute for Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark., Moser C; Department of Plastic Surgery, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark. |
| المصدر: | Pathogens and disease [Pathog Dis] 2020 Jul 01; Vol. 78 (5). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 101595366 Publication Model: Print Cited Medium: Internet ISSN: 2049-632X (Electronic) Linking ISSN: 2049632X NLM ISO Abbreviation: Pathog Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Oxford Oxford University Press Original Publication: [Malden, MA] : Wiley-Blackwell, [2013]- |
| مواضيع طبية MeSH: | Biofilms/*drug effects , Calgranulin A/*physiology , Ciprofloxacin/*pharmacology , Pseudomonas aeruginosa/*drug effects , Pseudomonas aeruginosa/*physiology , Wound Infection/*immunology , Wound Infection/*microbiology, Animals ; Anti-Bacterial Agents/pharmacology ; Chronic Disease ; Colony Count, Microbial ; Cytokines/metabolism ; Disease Models, Animal ; Drug Synergism ; Female ; Host-Pathogen Interactions ; Intercellular Signaling Peptides and Proteins/metabolism ; Mice ; Mice, Inbred BALB C ; Pseudomonas Infections/immunology ; Pseudomonas Infections/microbiology |
| مستخلص: | The majority of chronic wounds are associated with bacterial biofilms recalcitrant to antibiotics and host responses. Immunomodulatory S100A8/A9 is suppressed in Pseudomonas aeruginosa biofilm infected wounds. We aimed at investigating a possible additive effect between S100A8/A9 and ciprofloxacin against biofilms. Materials/methods: Thirty-two mice were injected with alginate-embedded P. aeruginosa following a third-degree burn. The mice were randomized into four groups receiving combination ciprofloxacin and S100A8/A9 or monotherapy ciprofloxacin, S100A8/A9 or a placebo and evaluated by host responses and quantitative bacteriology in wounds. In addition, in vitro checkerboard analysis was performed, with P. aeruginosa and ascending S100A8/A9 and ciprofloxacin concentrations. Results: S100A8/A9 augmented the effect of ciprofloxacin in vivo by lowering the bacterial quantity compared to the placebo arm and the two monointervention groups (P < 0.0001). S100A8 and 100A9 were increased in the double-treated group as compared to the monointervention groups (P = 0.032, P = 0.0023). Tissue inhibitor of metalloproteinases-1 and keratinocyte\chemokine chemoattractant-1 were increased in the double-intervention group compared to the S100A8/A9 group (P = 0.050, P = 0.050). No in vitro synergism was detected. Conclusion: The observed ciprofloxacin-augmenting effect of S100A8/A9 in vivo was not confirmed by checkerboard analysis, indicating dependence on host cells for the S100A8/A9 effect. S100A8/A9 and ciprofloxacin is a promising therapy for optimizing chronic wound treatment. (© FEMS 2020.) |
| فهرسة مساهمة: | Keywords: Pseudomonas; biofilm; flouroquinolone |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Calgranulin A) 0 (Cytokines) 0 (Intercellular Signaling Peptides and Proteins) 0 (S100a8 protein, mouse) 5E8K9I0O4U (Ciprofloxacin) |
| تواريخ الأحداث: | Date Created: 20190523 Date Completed: 20210604 Latest Revision: 20210604 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/femspd/ftz027 |
| PMID: | 31116394 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2049-632X |
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| DOI: | 10.1093/femspd/ftz027 |