دورية أكاديمية
Novel MLST sequence types of pathogenic Leptospira spp.: Opening the black box of animal leptospirosis in Brazil.
| العنوان: | Novel MLST sequence types of pathogenic Leptospira spp.: Opening the black box of animal leptospirosis in Brazil. |
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| المؤلفون: | Jaeger LH; Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institut, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil. Electronic address: laurenhj@hotmail.com., Pestana CP; Laboratory of Recombinant Technology, Bio-Manguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil. Electronic address: cristiane.pinheiro@bio.fiocruz.br., Correia L; Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil. Electronic address: lucascorreia@id.uff.br., Carvalho-Costa FA; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institut, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil. Electronic address: carvalhocosta70@hotmail.com., Medeiros MA; Laboratory of Recombinant Technology, Bio-Manguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil. Electronic address: medeiros@bio.fiocruz.br., Lilenbaum W; Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil. Electronic address: wlilenbaum@id.uff.br. |
| المصدر: | Acta tropica [Acta Trop] 2019 Aug; Vol. 196, pp. 135-141. Date of Electronic Publication: 2019 May 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Basel. |
| مواضيع طبية MeSH: | Multilocus Sequence Typing*, Leptospira/*genetics , Leptospirosis/*veterinary, Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Brazil/epidemiology ; Gene Expression Regulation, Bacterial ; Genetic Variation ; Humans ; Leptospira/isolation & purification ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Phylogeny |
| مستخلص: | In the current context of the emergence of certain infectious diseases and discussion of the One Health concept for many of these, the study of leptospirosis - both in domestic and wild hosts - cannot be neglected. The study of animal leptospirosis has evolved in recent years. It has been demonstrated that the human-animal-environment interface is more important than previously thought. In the present study, 35 strains of five pathogenic Leptospira species were isolated from different animal species in Brazil and characterized by rrs, secY, and Multilocus Sequence Typing (MLST) sequencing. Phylogenetic inferences were performed and the molecular diversity of the populations (intra- and inter-population levels) was evaluated. Among the five studied species, 18 different sequence types (STs) were found (22 new alleles and 11 new STs). eBURST analysis revealed two clonal complexes (CCs) and seven singletons. A high genetic diversity was demonstrated (H = 0.954 ± 0.017), mainly for the L. santarosai population (H = 0.942 ± 0.034, n = 20). The same strain was identified in different host species, as well as strains with zoonotic potential circulating in the country. Although the difficulty of culturing Leptospira strains is well known, the high variability of the strains found in Brazil highlights the importance of animals in maintaining the biological cycle of the bacterium in nature. Moreover, the selection of autochthonous strains for the development of vaccines becomes a challenge. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Animal leptospirosis; Genotyping; Leptospira; MLST; secY |
| المشرفين على المادة: | 0 (Bacterial Proteins) |
| تواريخ الأحداث: | Date Created: 20190524 Date Completed: 20191104 Latest Revision: 20191104 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.actatropica.2019.05.025 |
| PMID: | 31121146 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6254 |
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| DOI: | 10.1016/j.actatropica.2019.05.025 |