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Germ cell-less hybrid fish: ideal recipient for spermatogonial transplantation for the rapid production of donor-derived sperm†.

التفاصيل البيبلوغرافية
العنوان: Germ cell-less hybrid fish: ideal recipient for spermatogonial transplantation for the rapid production of donor-derived sperm†.
المؤلفون: Xu D; Marine Fishery Institute of Zhejiang Province, Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhoushan, Zhejiang Province, PR China.; Division of Fisheries Resource Sciences, Faculty of Fisheries, Kagoshima University, Shimoarata 4-50-20, Kagoshima City, Japan., Yoshino T; Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, Japan., Konishi J; Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, Japan., Yoshikawa H; Department of Applied Aquabiology, National Fisheries University, Japan Fisheries Research and Education Agency, 2-7-1 Nagata-Honmachi, Shimonoseki, Japan., Ino Y; Department of Applied Aquabiology, National Fisheries University, Japan Fisheries Research and Education Agency, 2-7-1 Nagata-Honmachi, Shimonoseki, Japan., Yazawa R; Department of Marine Bioscience, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, Japan., Dos Santos Nassif Lacerda SM; Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Brazil., de França LR; Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Takeuchi Y; Division of Fisheries Resource Sciences, Faculty of Fisheries, Kagoshima University, Shimoarata 4-50-20, Kagoshima City, Japan.; The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
المصدر: Biology of reproduction [Biol Reprod] 2019 Aug 01; Vol. 101 (2), pp. 492-500.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE
أسماء مطبوعة: Publication: 2017- : New York : Oxford University Press
Original Publication: Champaign, Ill. : Society for the Study of Reproduction
مواضيع طبية MeSH: Hybridization, Genetic*, Fishes/*genetics , Spermatogonia/*transplantation , Spermatozoa/*physiology, Animals ; Cell Transplantation ; Fishes/physiology ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Male ; Semen/cytology
مستخلص: An interspecific hybrid marine fish that developed a testis-like gonad without any germ cells, i.e., a germ cell-less gonad, was produced by hybridizing a female blue drum Nibea mitsukurii with a male white croaker Pennahia argentata. In this study, we evaluated the suitability of the germ cell-less fish as a recipient by transplanting donor testicular cells directly into the gonads through the urogenital papilla. The donor testicular cells were collected from hemizygous transgenic, green fluorescent protein (gfp) (+/-) blue drum, and transplanted into the germ cell-less gonads of the 6-month-old adult hybrid croakers. Fluorescent and histological observations showed the colonization, proliferation, and differentiation of transplanted spermatogonial cells in the gonads of hybrid croakers. The earliest production of spermatozoa in a hybrid recipient was observed at 7 weeks post-transplantation (pt), and 10% of the transplanted recipients produced donor-derived gfp-positive spermatozoa by 25 weeks pt. Sperm from the hybrid recipients were used to fertilize eggs from wild-type blue drums, and approximately 50% of the resulting offspring were gfp-positive, suggesting that all offspring originated from donor-derived sperm that were produced in the transplanted gfp (+/-) germ cells. To the best of our knowledge, this is the first report of successful spermatogonial transplantation using a germ cell-less adult fish as a recipient. This transplantation system has considerable advantages, such as the use of comparatively simple equipment and procedures, and rapid generation of donor-derived spermatogenesis and offspring, and presents numerous applications in commercial aquaculture.
(© The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction.)
فهرسة مساهمة: Keywords: fish reproduction; gametogenesis; male infertility; spermatogenesis; spermatogonia; surrogate broodstock; testis; transplantation
المشرفين على المادة: 0 (enhanced green fluorescent protein)
147336-22-9 (Green Fluorescent Proteins)
تواريخ الأحداث: Date Created: 20190528 Date Completed: 20200929 Latest Revision: 20200929
رمز التحديث: 20221213
DOI: 10.1093/biolre/ioz045
PMID: 31132090
قاعدة البيانات: MEDLINE
الوصف
تدمد:1529-7268
DOI:10.1093/biolre/ioz045