دورية أكاديمية
Host and MTB genome encoded miRNA markers for diagnosis of tuberculosis.
| العنوان: | Host and MTB genome encoded miRNA markers for diagnosis of tuberculosis. |
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| المؤلفون: | Chakrabarty S; Department of Cell and Molecular Biology, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India., Kumar A; Department of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India., Raviprasad K; Department of Cell and Molecular Biology, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India., Mallya S; Department of Bioinformatics, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India., Satyamoorthy K; Department of Cell and Molecular Biology, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India. Electronic address: ksatyamoorthy@manipal.edu., Chawla K; Department of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India. Electronic address: kiran.chawla@manipal.edu. |
| المصدر: | Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2019 May; Vol. 116, pp. 37-43. Date of Electronic Publication: 2019 Apr 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Edinburgh, New York : Churchill Livingstone, c2001- |
| مواضيع طبية MeSH: | Bacteriological Techniques* , Polymerase Chain Reaction*, Cell-Free Nucleic Acids/*genetics , MicroRNAs/*genetics , Mycobacterium tuberculosis/*genetics , RNA, Bacterial/*genetics , Tuberculosis/*diagnosis, Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; Cell-Free Nucleic Acids/blood ; Female ; Genetic Markers ; Host-Pathogen Interactions ; Humans ; India ; Male ; MicroRNAs/blood ; Middle Aged ; Predictive Value of Tests ; RNA, Bacterial/blood ; Tuberculosis/blood ; Tuberculosis/genetics ; Tuberculosis/microbiology ; Young Adult |
| مستخلص: | MicroRNAs (miRNAs) are a class of noncoding RNA molecules which are involved in various cellular and physiological processes. Previously, studies have identified several miRNAs that are potential diagnostic biomarkers for various infectious diseases including tuberculosis. We have performed small RNA sequencing using the Ion Torrent PGM platform in extra pulmonary tuberculosis (EPTB) subject's serum samples to identify circulating miRNAs during mycobacterium tuberculosis (MTB) infection. Our analysis identified 20 circulating miRNAs upregulated and 5 miRNAs downregulated during MTB infection in patient's serum. In addition, we have identified 6 MTB genome encoded miRNAs upregulated in EPTB patient's serum samples. Taqman based qRT-PCR analysis of host-genome encoded (hsa-miR-146a-5p and hsa-miR-125b-5p) and MTB genome encoded (MTB-miR5) miRNAs showed increased expression in a cohort of 52 healthy, pulmonary tuberculosis (PTB) and extra pulmonary tuberculosis (EPTB) patients serum samples. Our study identified for the first time a panel of host and MTB genome specific differentially expressed circulating miRNAs in serum samples of an Indian patient cohort with tuberculosis infection with a potential as a non-invasive diagnostic biomarker for tuberculosis infection. (Copyright © 2019. Published by Elsevier Ltd.) |
| المشرفين على المادة: | 0 (Cell-Free Nucleic Acids) 0 (Genetic Markers) 0 (MicroRNAs) 0 (RNA, Bacterial) |
| تواريخ الأحداث: | Date Created: 20190603 Date Completed: 20200114 Latest Revision: 20200114 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.tube.2019.04.002 |
| PMID: | 31153517 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-281X |
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| DOI: | 10.1016/j.tube.2019.04.002 |