دورية أكاديمية
Beyond diphtheria toxin: cytotoxic proteins of Corynebacterium ulcerans and Corynebacterium diphtheriae.
| العنوان: | Beyond diphtheria toxin: cytotoxic proteins of Corynebacterium ulcerans and Corynebacterium diphtheriae. |
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| المؤلفون: | Weerasekera D; > Microbiology Division, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany., Möller J; > Microbiology Division, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany., Kraner ME; Biochemistry Division, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany., Azevedo Antunes C; > Microbiology Division, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany.; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance-LDCIC, Faculty of Medical Sciences, Rio de Janeiro State University, Rio de Janeiro, Brazil., Mattos-Guaraldi AL; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance-LDCIC, Faculty of Medical Sciences, Rio de Janeiro State University, Rio de Janeiro, Brazil., Burkovski A; > Microbiology Division, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 2019 Aug; Vol. 165 (8), pp. 876-890. Date of Electronic Publication: 2019 Jun 04. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-2080 (Electronic) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | Corynebacterium/*genetics , Corynebacterium diphtheriae/*genetics , Cytotoxins/*biosynthesis , Exotoxins/*genetics , Virulence Factors/*genetics, Animals ; Bacterial Proteins/biosynthesis ; Corynebacterium/pathogenicity ; Corynebacterium Infections/microbiology ; Corynebacterium diphtheriae/pathogenicity ; Cytotoxins/genetics ; Diphtheria/microbiology ; Diphtheria Toxin ; Exotoxins/biosynthesis ; Humans ; Virulence Factors/biosynthesis |
| مستخلص: | Diphtheria toxin is one of the best investigated bacterial toxins and the major virulence factor of toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans strains. However, also diphtheria toxin-free strains of these two species can cause severe infections in animals and humans, indicating the presence of additional virulence factors. In this study, we present a first characterization of two proteins with cytotoxic effect in corynebacteria. A putative ribosome-binding protein (AEG80717, CULC809_00177), first annotated in a genome sequencing project of C. ulcerans strain 809, was investigated in detail together with a homologous protein identified in C. diphtheriae strain HC04 (AEX80148, CDHC04_0155) in this study. The corresponding proteins show striking structural similarity to Shiga-like toxins. Interaction of wild-type, mutant and complementation as well as overexpression strains with invertebrate model systems and cell lines were investigated. Depending on the presence of the corresponding genes, detrimental effects were observed in vivo in two invertebrate model systems, Caenorhabditis elegans and Galleria mellonella, and on various animal and human epithelial and macrophage cell lines in vitro. Taken together, our results support the idea that pathogenicity of corynebacteria is a multifactorial process and that new virulence factors may influence the outcome of potentially fatal corynebacterial infections. |
| فهرسة مساهمة: | Keywords: diphtheria; Vero cells; exotoxin; ribosome-binding protein; toxigenic corynebacteria |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Cytotoxins) 0 (Diphtheria Toxin) 0 (Exotoxins) 0 (Virulence Factors) |
| SCR Organism: | Corynebacterium ulcerans |
| تواريخ الأحداث: | Date Created: 20190605 Date Completed: 20200605 Latest Revision: 20200826 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1099/mic.0.000820 |
| PMID: | 31162026 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2080 |
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| DOI: | 10.1099/mic.0.000820 |