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أعرض في EDS

Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus.

التفاصيل البيبلوغرافية
العنوان: Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus.
المؤلفون: Dedkov VG; Pasteur Institute, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saint-Petersburg, Russia; Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, Moscow, Russia. Electronic address: vgdedkov@yandex.ru., Magassouba N'; Laboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea., Safonova MV; Anti-Plague Center, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia., Naydenova EV; Russian Research Anti-Plague Institute «Microbe», Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saratov, Russia., Ayginin AA; Central Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia., Soropogui B; Laboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea., Kourouma F; Laboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea., Camara AB; Laboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea., Camara J; Laboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea., Kritzkiy AA; Russian Research Anti-Plague Institute «Microbe», Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saratov, Russia., Tuchkov IV; Russian Research Anti-Plague Institute «Microbe», Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saratov, Russia., Shchelkanov MY; Far Eastern Federal University, Vladivostok, Russia., Maleev VV; Central Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
المصدر: Journal of virological methods [J Virol Methods] 2019 Sep; Vol. 271, pp. 113674. Date of Electronic Publication: 2019 Jun 03.
نوع المنشور: Evaluation Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
مواضيع طبية MeSH: Lassa Fever/*diagnosis , Lassa virus/*isolation & purification , RNA, Viral/*isolation & purification , Real-Time Polymerase Chain Reaction/*methods, Adolescent ; Adult ; Animals ; Child ; DNA Primers/genetics ; DNA Probes/genetics ; Female ; Guinea ; Humans ; Lassa Fever/blood ; Limit of Detection ; Male ; Middle Aged ; Murinae/virology ; Sensitivity and Specificity ; Young Adult
مستخلص: Lassa fever is a severe viral hemorrhagic illness caused by Lassa virus. Based on estimates, the number of LASV infections ranges from 300,000 to 500,000 cases in endemic areas with a fatality rate of 1%. Development of fast and sensitive tools for the control and prevention of Lassa virus infection as well as for clinical diagnostics of Lassa fever are crucial. Here we reported development and evaluation of a one-step quantitative RT-qPCR assay for the Lassa virus detection - LASV-Fl. This assay is suitable for the detection of lineages I-IV of Lassa virus. The limit of detection of the assay ranged from 10 3 copies/ml to 10 5 copies/ml and has 96.4% diagnostic sensitivity, whereas analytical and diagnostic specificities both were 100%. Serum, whole blood and tissue are suitable for use with the assay. The assay contains all the necessary components to perform the analysis, including an armored positive control (ARC+) and an armored internal control (IC). The study was done during the mission of specialized anti-epidemic team of the Russian Federation (SAET) in the Republic of Guinea in 2015-2018. Based on sequencing data, LASV-specific assay was developed using synthetic MS2-phage-based armored RNA particles, RNA from Lassa virus strain Josiah, and further, evaluated in field conditions using samples from patients and Mastomys natalensis rodents.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
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فهرسة مساهمة: Keywords: Guinea; Lassa fever; Lassa virus; quantitative RT-PCR
المشرفين على المادة: 0 (DNA Primers)
0 (DNA Probes)
0 (RNA, Viral)
تواريخ الأحداث: Date Created: 20190607 Date Completed: 20200603 Latest Revision: 20210109
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7113850
DOI: 10.1016/j.jviromet.2019.113674
PMID: 31170468
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0984
DOI:10.1016/j.jviromet.2019.113674