دورية أكاديمية

Determination of sialic acid levels by using surface-enhanced Raman spectroscopy in periodontitis and gingivitis.

التفاصيل البيبلوغرافية
العنوان: Determination of sialic acid levels by using surface-enhanced Raman spectroscopy in periodontitis and gingivitis.
المؤلفون: Hernández-Cedillo A; Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, San Luis Potosí, México., García-Valdivieso MG; Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, San Luis Potosí, México., Hernández-Arteaga AC; Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, San Luis Potosí, México., Patiño-Marín N; Facultad de Estomatología, Universidad Autónoma de San Luís Potosí, San Luis Potosí, México., Vértiz-Hernández ÁA; Coordinación Académica Región Altiplano de la Universidad Autónoma de San Luís Potosí, Matehuala, S.L.P., México., José-Yacamán M; Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, San Luis Potosí, México.; Department of Physics and Astronomy, University of Texas at San Antonio, San Antonio, Texas, USA., Navarro-Contreras HR; Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luís Potosí, San Luis Potosí, México.
المصدر: Oral diseases [Oral Dis] 2019 Sep; Vol. 25 (6), pp. 1627-1633. Date of Electronic Publication: 2019 Jul 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Munksgaard Country of Publication: Denmark NLM ID: 9508565 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1601-0825 (Electronic) Linking ISSN: 1354523X NLM ISO Abbreviation: Oral Dis
أسماء مطبوعة: Publication: 2001- : Copenhagen, Denmark : Munksgaard
Original Publication: Houndmills, Basingstoke, Hampshire, UK : Stockton Press, c1995-
مواضيع طبية MeSH: N-Acetylneuraminic Acid/*analysis , Periodontitis/*diagnosis , Saliva/*chemistry, Adult ; Female ; Gingivitis ; Humans ; Male ; Middle Aged ; N-Acetylneuraminic Acid/chemistry ; Periodontal Index ; Saliva/metabolism ; Spectrum Analysis, Raman
مستخلص: Objectives: To compare the sialic acid (SA) levels in saliva among periodontitis-affected, gingivitis and control patients.
Methods: The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingivitis; and (3) 33 subjects with periodontitis. The oral parameters examined were as follows: (a) Simplified Oral Hygiene Index; (b) Calculus Index; (c) Gingival Index; (d) probing pocket depth; and (e) level of epithelial attachment. SA levels in saliva were measured by means of surface-enhanced Raman spectroscopy (SERS). This method has demonstrated the capacity to detect extremely low concentrations of molecules. The spectrum was calibrated using analytical reagent SA.
Results: The obtained median values for SA concentrations were 5.98, 7.32, and 17.12 mg/dl for control, gingivitis, and periodontitis patients, respectively.
Conclusions: Our measurements by SERS corroborate that in periodontitis-affected patients, the SA concentration is larger than their concentrations in either control or gingivitis patients. This confirms previous reports and opens the possibility of using SERS as a diagnostic tool.
(© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.)
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معلومات مُعتمدة: FAI-UASLP; 446208 Consejo Nacional de Ciencia y Tecnología; 2015-01-986 Consejo Nacional de Ciencia y Tecnología; #AX-1615 Welch Foundation; Laboratorio Nacional de Análisis Físicos, Químicos y Biológicos-UASLP; Clínica de Periodoncia, Facultad de Estomatología, UASLP
فهرسة مساهمة: Keywords: Ag nanoparticles; gingivitis; periodontitis; sialic acid; surface-enhanced Raman
المشرفين على المادة: GZP2782OP0 (N-Acetylneuraminic Acid)
تواريخ الأحداث: Date Created: 20190610 Date Completed: 20191230 Latest Revision: 20200108
رمز التحديث: 20221213
DOI: 10.1111/odi.13141
PMID: 31177594
قاعدة البيانات: MEDLINE
الوصف
تدمد:1601-0825
DOI:10.1111/odi.13141