دورية أكاديمية
Regulation of hsnT , nodF and nodE genes in Rhizobium tropici CIAT 899 and their roles in the synthesis of Nod factors and in the symbiosis.
| العنوان: | Regulation of hsnT , nodF and nodE genes in Rhizobium tropici CIAT 899 and their roles in the synthesis of Nod factors and in the symbiosis. |
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| المؤلفون: | Gomes DF; Embrapa Soja, C.P. 231, 86001-970 Londrina, Paraná, Brazil., Tullio LD; Embrapa Soja, C.P. 231, 86001-970 Londrina, Paraná, Brazil.; Universidade Estadual de Londrina, Dept. Bioquímica e Biotecnologia, C.P. 60001, 86051-990, Londrina, Paraná, Brazil., Del Cerro P; Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes, 6, 41012 Sevilla, Spain., Nakatani AS; Embrapa Soja, C.P. 231, 86001-970 Londrina, Paraná, Brazil., Rolla-Santos AAP; Embrapa Soja, C.P. 231, 86001-970 Londrina, Paraná, Brazil., Gil-Serrano A; Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, Calle Profesor García González, 8, 41012 Sevilla, Spain., Megías M; Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes, 6, 41012 Sevilla, Spain., Ollero FJ; Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes, 6, 41012 Sevilla, Spain., Hungria M; Embrapa Soja, C.P. 231, 86001-970 Londrina, Paraná, Brazil.; Universidade Estadual de Londrina, Dept. Bioquímica e Biotecnologia, C.P. 60001, 86051-990, Londrina, Paraná, Brazil. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 2019 Sep; Vol. 165 (9), pp. 990-1000. Date of Electronic Publication: 2019 Jun 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-2080 (Electronic) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | Bacterial Proteins/*genetics , Plant Root Nodulation/*genetics , Rhizobium tropici/*genetics, Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Nitrogen Fixation/physiology ; Phaseolus/microbiology ; Symbiosis/genetics |
| مستخلص: | Rhizobium tropici strain CIAT 899 possesses outstanding agronomic properties as it displays tolerance to environmental stresses, a broad host range and high effectiveness in fixing nitrogen with the common bean ( Phaseolus vulgaris L.); in addition, it carries intriguing features such as five copies of the regulatory nodD gene, and the capacity to synthesize a variety of nodulation factors (NFs), even in a flavonoid-independent manner, when submitted to abiotic stresses. However, the roles of several nod genes of the repertoire of CIAT 899 remain to be determined. In this study, we obtained mutants for the hsnT , nodF and nodE genes of CIAT 899 and investigated their expression, NF structures and symbiotic properties. Either in the presence of the flavonoid apigenin, or of salt the expression of hsnT , nodF and nodE in wild-type CIAT 899 was highly up-regulated in comparison to the mutants of all five copies of nodD , indicating the roles that regulatory nodD genes play in the activation of hsnT , nodF and nodE ; however, NodD1 was recognized as the main inducer. In total, 29 different NF structures were synthesized by wild-type CIAT 899 induced by apigenin, and 36 when induced by salt, being drastically reduced by mutations in hsnT , nodF and nodE , especially under osmotic stress, with specific changes related to each gene, indicating that the three genes participate in the synthesis of NFs. Mutations in hsnT , nodF and nodE affected differently symbiotic performance (nodule number and shoot dry weight), according to the host plant. Our results indicate that the expression of hsnT , nodF and nodE genes of CIAT 899 is mediated by nodD genes, and although these three genes do not belong to the main set of genes controlling nodulation, they contribute to the synthesis of NFs that will impact symbiotic performance and host specificity. |
| فهرسة مساهمة: | Keywords: Biological nitrogen fixation; Host-specific genes; LCO; Nodulation; Symbiosis; nod genes |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (NodD protein, Bacteria) |
| تواريخ الأحداث: | Date Created: 20190612 Date Completed: 20200514 Latest Revision: 20200826 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1099/mic.0.000824 |
| PMID: | 31184576 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2080 |
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| DOI: | 10.1099/mic.0.000824 |