دورية أكاديمية
Thiamine and benfotiamine protect neuroblastoma cells against paraquat and β-amyloid toxicity by a coenzyme-independent mechanism.
| العنوان: | Thiamine and benfotiamine protect neuroblastoma cells against paraquat and β-amyloid toxicity by a coenzyme-independent mechanism. |
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| المؤلفون: | Sambon M; Laboratory of Neurophysiology, GIGA-Neurosciences, University of Liège, Liège, Belgium., Napp A; Laboratory for the Analysis of Medicines, CIRM, Department of Pharmacy, University of Liège, Liège, Belgium., Demelenne A; Laboratory for the Analysis of Medicines, CIRM, Department of Pharmacy, University of Liège, Liège, Belgium., Vignisse J; Laboratory of Neurophysiology, GIGA-Neurosciences, University of Liège, Liège, Belgium., Wins P; Laboratory of Neurophysiology, GIGA-Neurosciences, University of Liège, Liège, Belgium., Fillet M; Laboratory for the Analysis of Medicines, CIRM, Department of Pharmacy, University of Liège, Liège, Belgium., Bettendorff L; Laboratory of Neurophysiology, GIGA-Neurosciences, University of Liège, Liège, Belgium. |
| المصدر: | Heliyon [Heliyon] 2019 May 14; Vol. 5 (5), pp. e01710. Date of Electronic Publication: 2019 May 14 (Print Publication: 2019). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Elsevier Ltd, [2015]- |
| مستخلص: | Background: Benfotiamine (BFT) is a synthetic thiamine precursor with high bioavailability. It is efficient in treating complications of type 2 diabetes and has beneficial effects in mouse models of neurodegenerative diseases. The mechanism of action of BFT remains unknown, though it is sometimes suggested that it may be linked to increased thiamine diphosphate (ThDP) coenzyme function. Methods: We used a mouse neuroblastoma cell line (Neuro2a) grown in thiamine-restricted medium. The cells were stressed by exposure to paraquat (PQ) or amyloid β Results: At 50 μM, BFT protects the cells against PQ and amyloid β Conclusions: BFT, SuBT and thiamine all protect the cells against oxidative stress, suggesting an antioxidant effect of thiamine. Our results are not in favor of a direct ROS scavenging effect of thiamine but rather an indirect effect possibly mediated by some antioxidant signaling pathway. It is however not clear whether this effect is due to thiamine itself, its thiol form or an unknown metabolite. General Significance: Our results suggest a role of thiamine in protection against oxidative stress, independent of the coenzyme function of thiamine diphosphate. |
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| فهرسة مساهمة: | Keywords: ARE, antioxidant response element; BFT, benfotiamine; Cell biology; FBS, fetal bovine serum; Neuroscience; O-BT, O-benzoylthiamine; PQ, paraquat; ROS, reactive oxygen species; S-BT, S-benzoylthiamine; SuBT, sulbutiamine; TPK, thiamine pyrophosphokinase; ThDP, thiamine diphosphate; ThMP, thiamine monophosphate |
| تواريخ الأحداث: | Date Created: 20190614 Latest Revision: 20220408 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6520661 |
| DOI: | 10.1016/j.heliyon.2019.e01710 |
| PMID: | 31193162 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2405-8440 |
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| DOI: | 10.1016/j.heliyon.2019.e01710 |