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Development of a loop-mediated isothermal amplification assay for rapid Helicobacter pylori detection.

التفاصيل البيبلوغرافية
العنوان: Development of a loop-mediated isothermal amplification assay for rapid Helicobacter pylori detection.
المؤلفون: Horiuchi S; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan; Department of Public Health Nursing, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan. Electronic address: saorih@naramed-u.ac.jp., Nakano R; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan., Nakano A; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan., Hishiya N; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan; Department of Infectious Diseases, Minami-Nara General Medical Center, 8-1 Fukugami, Yoshino-gun, Oyodo, Nara 638-8551, Japan., Uno K; Department of Infectious Diseases, Minami-Nara General Medical Center, 8-1 Fukugami, Yoshino-gun, Oyodo, Nara 638-8551, Japan., Suzuki Y; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan., Tanouchi A; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan., Kakuta N; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan., Masui T; Department of otolaryngology - Head and Neck Surgery, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan., Jojima N; Department of Public Health Nursing, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan., Yano H; Department of Microbiology and Infectious Diseases, Nara Medical University, 840 Shijo-Cho, Kashihara, Nara 634-8521, Japan.
المصدر: Journal of microbiological methods [J Microbiol Methods] 2019 Aug; Vol. 163, pp. 105653. Date of Electronic Publication: 2019 Jun 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983-
مواضيع طبية MeSH: Molecular Diagnostic Techniques*/methods , Nucleic Acid Amplification Techniques*/methods, Helicobacter Infections/*diagnosis , Helicobacter pylori/*isolation & purification, Antigens, Bacterial/genetics ; Bacterial Proteins/genetics ; Feces/microbiology ; Genes, Bacterial ; Helicobacter pylori/genetics
مستخلص: Infection with cagA-positive Helicobacter pylori is associated with gastric cancer. Molecular techniques are vital for accurate H. pylori diagnosis. We developed a loop-mediated isothermal amplification (LAMP) for detecting the H. pylori cagA gene and evaluated its use for clinical diagnosis. A LAMP primer set was designed to recognize the homologous regions of cagA gene sequences of 6 H. pylori strains. LAMP sensitivity was evaluated with serial dilutions of H. pylori ATCC 43504 and fecal specimens; specificity was evaluated with H. pylori ATCC 49396 and CIP 104086. The LAMP sensitivity for H. pylori specimens was 10 -1  cfu/tube (reaction time, 37 min), which was 10-fold more sensitive than polymerase chain reaction. LAMP was also highly sensitive and rapid for fecal specimens. It detected cagA gene from ATCC 49396 and CIP 104086. The findings suggest LAMP can be used for diagnosing and screening of H. pylori infections to decrease gastric cancer incidence.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Helicobacter pylori; Loop-mediated isothermal amplification; Rapid detection
المشرفين على المادة: 0 (Antigens, Bacterial)
0 (Bacterial Proteins)
0 (cagA protein, Helicobacter pylori)
SCR Protocol: LAMP assay
تواريخ الأحداث: Date Created: 20190615 Date Completed: 20200615 Latest Revision: 20200615
رمز التحديث: 20240628
DOI: 10.1016/j.mimet.2019.105653
PMID: 31199937
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-8359
DOI:10.1016/j.mimet.2019.105653