دورية أكاديمية
أعرض في EDS

Anterior-enriched filopodia create the appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes.

التفاصيل البيبلوغرافية
العنوان: Anterior-enriched filopodia create the appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes.
المؤلفون: Hirani N; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK., Illukkumbura R; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK., Bland T; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.; Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UK., Mathonnet G; Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, UMR7104, Institut National de la Santé et de la Recherche Médicale, U1258, and Université de Strasbourg, 67404 Illkirch, France., Suhner D; Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, UMR7104, Institut National de la Santé et de la Recherche Médicale, U1258, and Université de Strasbourg, 67404 Illkirch, France., Reymann AC; Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, UMR7104, Institut National de la Santé et de la Recherche Médicale, U1258, and Université de Strasbourg, 67404 Illkirch, France., Goehring NW; The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK nate.goehring@crick.ac.uk.; Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UK.; MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
المصدر: Journal of cell science [J Cell Sci] 2019 Jul 15; Vol. 132 (14). Date of Electronic Publication: 2019 Jul 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge : Company of Biologists
Original Publication: London.
مواضيع طبية MeSH: Caenorhabditis elegans/*embryology , Caenorhabditis elegans/*metabolism , Membrane Microdomains/*metabolism , Pseudopodia/*metabolism , Zygote/*metabolism, Actins/metabolism ; Animals ; Embryo, Nonmammalian/cytology ; Embryo, Nonmammalian/metabolism ; Green Fluorescent Proteins/metabolism
مستخلص: The association of molecules within membrane microdomains is critical for the intracellular organization of cells. During polarization of the C. elegans zygote, both polarity proteins and actomyosin regulators associate within dynamic membrane-associated foci. Recently, a novel class of asymmetric membrane-associated structures was described that appeared to be enriched in phosphatidylinositol 4,5-bisphosphate (PIP 2 ), suggesting that PIP 2 domains could constitute signaling hubs to promote cell polarization and actin nucleation. Here, we probe the nature of these domains using a variety of membrane- and actin cortex-associated probes. These data demonstrate that these domains are filopodia, which are stimulated transiently during polarity establishment and accumulate in the zygote anterior. The resulting membrane protrusions create local membrane topology that quantitatively accounts for observed local increases in the fluorescence signal of membrane-associated molecules, suggesting molecules are not selectively enriched in these domains relative to bulk membrane and that the PIP 2 pool as revealed by PH PLCδ1 simply reflects plasma membrane localization. Given the ubiquity of 3D membrane structures in cells, including filopodia, microvilli and membrane folds, similar caveats are likely to apply to analysis of membrane-associated molecules in a broad range of systems.
Competing Interests: Competing interestsThe authors declare no competing or financial interests.
(© 2019. Published by The Company of Biologists Ltd.)
References: Curr Biol. 1999 Jul 15;9(14):738-45. (PMID: 10421575)
Genetics. 2001 Mar;157(3):1217-26. (PMID: 11238406)
Mol Biol Cell. 2002 Sep;13(9):3257-67. (PMID: 12221130)
Nature. 2003 Jan 16;421(6920):231-7. (PMID: 12529635)
Dev Cell. 2004 Sep;7(3):413-24. (PMID: 15363415)
Curr Biol. 2005 Dec 20;15(24):2222-9. (PMID: 16360684)
Nat Cell Biol. 2006 Sep;8(9):978-85. (PMID: 16921365)
Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14976-81. (PMID: 17848508)
Plant J. 2008 Jan;53(1):186-96. (PMID: 17931350)
BMC Dev Biol. 2007 Dec 24;7:142. (PMID: 18157918)
Nat Methods. 2008 Feb;5(2):159-61. (PMID: 18176568)
Nat Rev Mol Cell Biol. 2008 Jun;9(6):446-54. (PMID: 18464790)
Nat Methods. 2008 Jul;5(7):605-7. (PMID: 18536722)
Dev Cell. 2008 Aug;15(2):198-208. (PMID: 18694560)
Dev Cell. 2009 Jun;16(6):889-900. (PMID: 19531359)
Mol Biol Cell. 2010 Jan 15;21(2):266-77. (PMID: 19923324)
Biophys J. 2010 Oct 20;99(8):2443-52. (PMID: 20959084)
J Cell Biol. 2011 May 2;193(3):583-94. (PMID: 21518794)
PLoS One. 2011;6(9):e24656. (PMID: 21935434)
Cytoskeleton (Hoboken). 2011 Oct;68(10):578-87. (PMID: 21948789)
Nat Cell Biol. 2011 Oct 09;13(11):1361-7. (PMID: 21983565)
Nature. 2011 Oct 23;479(7374):552-5. (PMID: 22020284)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
Biol Open. 2012 Sep 15;1(9):857-62. (PMID: 23213479)
Physiol Rev. 2013 Jul;93(3):1019-137. (PMID: 23899561)
Nat Methods. 2014 Jun;11(6):677-82. (PMID: 24727651)
Dev Biol. 2014 Sep 15;393(2):209-226. (PMID: 24995797)
Exp Cell Res. 2014 Nov 1;328(2):258-66. (PMID: 25128809)
Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. (PMID: 25535392)
Cell. 2015 Apr 9;161(2):374-86. (PMID: 25799384)
J Biol Chem. 2015 Nov 6;290(45):26978-93. (PMID: 26396197)
Nat Cell Biol. 2015 Nov;17(11):1471-83. (PMID: 26479320)
J Cell Biol. 2015 Oct 26;211(2):273-86. (PMID: 26483556)
Nat Cell Biol. 2016 Jun;18(6):676-83. (PMID: 27159499)
Annu Rev Cell Dev Biol. 2016 Oct 6;32:143-171. (PMID: 27576122)
Elife. 2016 Oct 10;5:. (PMID: 27719759)
Elife. 2017 Jan 28;6:. (PMID: 28117665)
Chem Rev. 2017 Jun 14;117(11):7457-7477. (PMID: 28211677)
J Cell Biol. 2017 May 1;216(5):1371-1386. (PMID: 28400443)
Nat Cell Biol. 2017 Aug;19(8):988-995. (PMID: 28737772)
Dev Cell. 2017 Aug 21;42(4):400-415.e9. (PMID: 28781174)
Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID: 28829947)
Development. 2018 May 30;145(11):. (PMID: 29724757)
J Cell Biol. 2018 Dec 3;217(12):4230-4252. (PMID: 30275107)
Curr Biol. 2019 Jan 21;29(2):202-216.e7. (PMID: 30639111)
Curr Biol. 2019 Jun 17;29(12):1911-1923.e5. (PMID: 31155349)
J Neurosci Res. 1985;13(1-2):149-62. (PMID: 3973930)
Genetics. 1974 May;77(1):71-94. (PMID: 4366476)
Nature. 1997 Jun 5;387(6633):569-72. (PMID: 9177342)
J Cell Sci. 1998 Jul 30;111 ( Pt 14):2017-27. (PMID: 9645949)
معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; FC001086 United Kingdom CRUK_ Cancer Research UK; FC001086 United Kingdom MRC_ Medical Research Council; P40 OD010440 United States OD NIH HHS
فهرسة مساهمة: Keywords: C. elegans; Cell cortex; Cell polarity; Filopodia; PAR proteins; PIP2
المشرفين على المادة: 0 (Actins)
147336-22-9 (Green Fluorescent Proteins)
تواريخ الأحداث: Date Created: 20190622 Date Completed: 20200709 Latest Revision: 20220716
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6679585
DOI: 10.1242/jcs.230714
PMID: 31221727
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-9137
DOI:10.1242/jcs.230714