دورية أكاديمية
أعرض في EDS

Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta.

التفاصيل البيبلوغرافية
العنوان: Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta.
المؤلفون: Mehdi R; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Lamm CE; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Bodampalli Anjanappa R; Plant Biotechnology, Department of Biology, ETH Zurich, Zurich, Switzerland., Müdsam C; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Saeed M; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Klima J; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Kraner ME; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Ludewig F; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Knoblauch M; School of Biological Sciences, Washington State University, Pullman, WA, USA., Gruissem W; Plant Biotechnology, Department of Biology, ETH Zurich, Zurich, Switzerland.; Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung City, Taiwan., Sonnewald U; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany., Zierer W; Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.
المصدر: Journal of experimental botany [J Exp Bot] 2019 Oct 24; Vol. 70 (20), pp. 5559-5573.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 9882906 Publication Model: Print Cited Medium: Internet ISSN: 1460-2431 (Electronic) Linking ISSN: 00220957 NLM ISO Abbreviation: J Exp Bot Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, UK : Oxford University Press,
مواضيع طبية MeSH: Manihot/*metabolism , Phloem/*metabolism , Plant Roots/*metabolism, Biological Transport ; Esculin/metabolism ; Gene Expression Regulation, Plant ; Manihot/physiology ; Phloem/physiology ; Plant Proteins/metabolism ; Plant Roots/physiology ; Xylem/metabolism ; Xylem/physiology
مستخلص: Cassava (Manihot esculenta) is one of the most important staple food crops worldwide. Its starchy tuberous roots supply over 800 million people with carbohydrates. Yet, surprisingly little is known about the processes involved in filling of those vital storage organs. A better understanding of cassava carbohydrate allocation and starch storage is key to improving storage root yield. Here, we studied cassava morphology and phloem sap flow from source to sink using transgenic pAtSUC2::GFP plants, the phloem tracers esculin and 5(6)-carboxyfluorescein diacetate, as well as several staining techniques. We show that cassava performs apoplasmic phloem loading in source leaves and symplasmic unloading into phloem parenchyma cells of tuberous roots. We demonstrate that vascular rays play an important role in radial transport from the phloem to xylem parenchyma cells in tuberous roots. Furthermore, enzymatic and proteomic measurements of storage root tissues confirmed high abundance and activity of enzymes involved in the sucrose synthase-mediated pathway and indicated that starch is stored most efficiently in the outer xylem layers of tuberous roots. Our findings form the basis for biotechnological approaches aimed at improved phloem loading and enhanced carbohydrate allocation and storage in order to increase tuberous root yield of cassava.
(© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.)
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فهرسة مساهمة: Keywords: Apoplast; CFDA; SUC2; cassava; esculin; morphology; phloem; ray; starch; symplast
المشرفين على المادة: 0 (Plant Proteins)
1Y1L18LQAF (Esculin)
تواريخ الأحداث: Date Created: 20190625 Date Completed: 20200817 Latest Revision: 20231014
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6812707
DOI: 10.1093/jxb/erz297
PMID: 31232453
قاعدة البيانات: MEDLINE
الوصف
تدمد:1460-2431
DOI:10.1093/jxb/erz297