دورية أكاديمية
Molecular recognition of HIV-1 RNAs with branched peptides.
| العنوان: | Molecular recognition of HIV-1 RNAs with branched peptides. |
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| المؤلفون: | Peralta AN; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States., Dai Y; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States., Sherpa C; Basic Research Laboratory, National Cancer Institute, Frederick, MD, United States., Le Grice SFJ; Basic Research Laboratory, National Cancer Institute, Frederick, MD, United States., Santos WL; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States. Electronic address: santosw@vt.edu. |
| المصدر: | Methods in enzymology [Methods Enzymol] 2019; Vol. 623, pp. 373-400. Date of Electronic Publication: 2019 May 15. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | HIV-1/*metabolism , Peptides/*pharmacology , RNA, Viral/*metabolism, Binding Sites ; Drug Discovery/methods ; HIV Infections/virology ; HIV-1/chemistry ; High-Throughput Screening Assays/methods ; Humans ; Peptide Library ; Peptides/chemistry ; RNA, Viral/chemistry |
| مستخلص: | Targeting RNA offers the potential in many diseases of a therapeutic treatment. Due to its large surface area and ability to adopt different conformations, targeting RNA has proven challenging. Medium-sized branched peptides are of the size to competitively bind RNA while remaining cell permeable, stable in vivo, and non-toxic. Additionally, the ease in generating a large library followed by high-throughput screening provides a way to suggest a scaffold with high diversity that is capable of targeting the structure and sequence of RNA. The ability to select various types of amino acid modifications in the branched peptide allows for variable structures and interactions of the branched peptide but can result in too large a task if not approached properly. In this chapter, we discuss a strategy to selectively recognize RNAs of interest through high throughput screening of branched peptides, validation of hits and biophysical characterization, leading by example with our experience in targeting HIV-1 RNAs with branched peptides. (© 2019 Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | R01 GM093834 United States GM NIGMS NIH HHS; R25 GM072767 United States GM NIGMS NIH HHS; ZIA BC011824 United States ImNIH Intramural NIH HHS |
| فهرسة مساهمة: | Keywords: Boronic acids; Branched peptides; HIV-1; RRE RNA; Unnatural amino acids |
| المشرفين على المادة: | 0 (Peptide Library) 0 (Peptides) 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20190627 Date Completed: 20200519 Latest Revision: 20210612 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8195442 |
| DOI: | 10.1016/bs.mie.2019.04.021 |
| PMID: | 31239054 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1557-7988 |
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| DOI: | 10.1016/bs.mie.2019.04.021 |