دورية أكاديمية
Molecular recognition of HIV-1 RNAs with branched peptides.
العنوان: | Molecular recognition of HIV-1 RNAs with branched peptides. |
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المؤلفون: | Peralta AN; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States., Dai Y; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States., Sherpa C; Basic Research Laboratory, National Cancer Institute, Frederick, MD, United States., Le Grice SFJ; Basic Research Laboratory, National Cancer Institute, Frederick, MD, United States., Santos WL; Department of Chemistry and Center for Drug Discovery, Virginia Tech, Blacksburg, VA, United States. Electronic address: santosw@vt.edu. |
المصدر: | Methods in enzymology [Methods Enzymol] 2019; Vol. 623, pp. 373-400. Date of Electronic Publication: 2019 May 15. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0212271 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7988 (Electronic) Linking ISSN: 00766879 NLM ISO Abbreviation: Methods Enzymol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: New York, Academic Press. |
مواضيع طبية MeSH: | HIV-1/*metabolism , Peptides/*pharmacology , RNA, Viral/*metabolism, Binding Sites ; Drug Discovery/methods ; HIV Infections/virology ; HIV-1/chemistry ; High-Throughput Screening Assays/methods ; Humans ; Peptide Library ; Peptides/chemistry ; RNA, Viral/chemistry |
مستخلص: | Targeting RNA offers the potential in many diseases of a therapeutic treatment. Due to its large surface area and ability to adopt different conformations, targeting RNA has proven challenging. Medium-sized branched peptides are of the size to competitively bind RNA while remaining cell permeable, stable in vivo, and non-toxic. Additionally, the ease in generating a large library followed by high-throughput screening provides a way to suggest a scaffold with high diversity that is capable of targeting the structure and sequence of RNA. The ability to select various types of amino acid modifications in the branched peptide allows for variable structures and interactions of the branched peptide but can result in too large a task if not approached properly. In this chapter, we discuss a strategy to selectively recognize RNAs of interest through high throughput screening of branched peptides, validation of hits and biophysical characterization, leading by example with our experience in targeting HIV-1 RNAs with branched peptides. (© 2019 Elsevier Inc. All rights reserved.) |
References: | Nat Protoc. 2015 Nov;10(11):1643-69. (PMID: 26426499) Chem Biol. 2001 May;8(5):511-20. (PMID: 11358697) Science. 2007 Jun 22;316(5832):1759-61. (PMID: 17588934) J Am Chem Soc. 2011 Jun 29;133(25):9912-22. (PMID: 21585196) Biochemistry. 2011 Jun 7;50(22):5042-57. (PMID: 21553929) J Am Chem Soc. 2006 Mar 22;128(11):3496-7. (PMID: 16536504) Medchemcomm. 2016 Jul 1;7(7):1436-1440. (PMID: 27453773) Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3127-32. (PMID: 11248043) Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11931-6. (PMID: 19584251) Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):683-7. (PMID: 1992459) Curr Opin Struct Biol. 1996 Jun;6(3):322-6. (PMID: 8804835) J Biomed Sci. 2007 Sep;14(5):565-84. (PMID: 17520355) Org Biomol Chem. 2013 Oct 7;11(37):6263-71. (PMID: 23925474) Chem Rev. 2008 Apr;108(4):1171-224. (PMID: 18361529) Arch Biochem Biophys. 1999 May 15;365(2):175-85. (PMID: 10328810) J Biol Chem. 2001 Jan 19;276(3):1968-73. (PMID: 11056173) Bioorg Med Chem. 1997 Jun;5(6):1173-84. (PMID: 9222511) Science. 1996 Sep 13;273(5281):1547-51. (PMID: 8703216) Oncologist. 2003;8(6):508-13. (PMID: 14657528) Anal Chem. 2011 May 1;83(9):3548-54. (PMID: 21449540) J Am Chem Soc. 2009 Feb 18;131(6):2224-30. (PMID: 19199621) J Med Chem. 2018 Nov 8;61(21):9611-9620. (PMID: 30289719) Nucleic Acids Res. 2018 Nov 2;46(19):10432-10447. (PMID: 30102382) PLoS Pathog. 2011 May;7(5):e1002038. (PMID: 21625572) Oligonucleotides. 2007 Fall;17(3):275-90. (PMID: 17854268) Biochemistry. 2000 May 16;39(19):5630-41. (PMID: 10801313) Bioorg Med Chem Lett. 2001 May 7;11(9):1169-72. (PMID: 11354369) ACS Chem Biol. 2012 Jan 20;7(1):210-7. (PMID: 22003984) Annu Rev Biochem. 1995;64:593-620. (PMID: 7574494) Angew Chem Int Ed Engl. 2008;47(1):134-7. (PMID: 17987636) Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14047-52. (PMID: 12376617) Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11887-92. (PMID: 9342332) Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2505-10. (PMID: 8637904) Viruses. 2015 Jun 12;7(6):3053-75. (PMID: 26075509) Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):12997-3002. (PMID: 10557261) Biochemistry. 2003 Oct 7;42(39):11391-403. (PMID: 14516190) J Virol. 1999 May;73(5):4341-9. (PMID: 10196332) Nat Rev Genet. 2011 May;12(5):329-40. (PMID: 21499294) J Am Chem Soc. 2007 Apr 18;129(15):4514-5. (PMID: 17378563) Chem Commun (Camb). 2013 Mar 25;49(24):2436-8. (PMID: 23412370) Drug Discov Today. 2003 Apr 1;8(7):297-306. (PMID: 12654542) Mol Biosyst. 2009 Sep;5(9):1070-3. (PMID: 19668873) |
معلومات مُعتمدة: | R01 GM093834 United States GM NIGMS NIH HHS; R25 GM072767 United States GM NIGMS NIH HHS; ZIA BC011824 United States ImNIH Intramural NIH HHS |
فهرسة مساهمة: | Keywords: Boronic acids; Branched peptides; HIV-1; RRE RNA; Unnatural amino acids |
المشرفين على المادة: | 0 (Peptide Library) 0 (Peptides) 0 (RNA, Viral) |
تواريخ الأحداث: | Date Created: 20190627 Date Completed: 20200519 Latest Revision: 20210612 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC8195442 |
DOI: | 10.1016/bs.mie.2019.04.021 |
PMID: | 31239054 |
قاعدة البيانات: | MEDLINE |
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