دورية أكاديمية
Different vasodilator mechanisms in intermediate- and small-sized arteries from the hindlimb vasculature of the toad Rhinella marina .
| العنوان: | Different vasodilator mechanisms in intermediate- and small-sized arteries from the hindlimb vasculature of the toad Rhinella marina . |
|---|---|
| المؤلفون: | Cameron MS; Deakin University, School of Life and Environmental Sciences, Waurn Ponds Campus, Geelong, Victoria, Australia.; Discipline of Physiology, School of Medical Sciences, The University of Sydney, Sydney, New South Wales, Australia., Donald JA; Deakin University, School of Life and Environmental Sciences, Waurn Ponds Campus, Geelong, Victoria, Australia. |
| المصدر: | American journal of physiology. Regulatory, integrative and comparative physiology [Am J Physiol Regul Integr Comp Physiol] 2019 Sep 01; Vol. 317 (3), pp. R379-R385. Date of Electronic Publication: 2019 Jun 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901230 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1490 (Electronic) Linking ISSN: 03636119 NLM ISO Abbreviation: Am J Physiol Regul Integr Comp Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, Md. : American Physiological Society |
| مواضيع طبية MeSH: | Arteries/*physiology , Hindlimb/*blood supply , Vasodilation/*physiology, Animals ; Arteries/anatomy & histology ; Bufo marinus ; Female ; Humans ; Immunohistochemistry ; Male ; Nitric Oxide Synthase/chemistry ; Nitric Oxide Synthase/metabolism ; Nitroarginine/pharmacology ; Oxadiazoles ; Quinoxalines ; Signal Transduction |
| مستخلص: | In this study, myography was used to determine the effect of arterial size on nitric oxide (NO) vasodilatory mechanisms in the hindlimb vasculature of the toad Rhinella marina . Immunohistochemical analysis showed NO synthase (NOS) 1 immunoreactivity in perivascular nitrergic nerves in the iliac and sciatic arteries. Furthermore, NOS3 immunoreactivity was observed in the vascular smooth muscle of the sciatic artery, but not the endothelium. Acetylcholine (ACh) was used to facilitate intracellular Ca 2+ signaling to activate vasodilatory pathways in the arteries. In the iliac artery, ACh-mediated vasodilation was abolished by blockade of the soluble guanylate cyclase pathway with the soluble guanylate cyclase inhibitor ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, 10 -5 M) and blockade of the prostaglandin signaling pathway with indomethacin (10 -5 M). Furthermore, disruption of the endothelium had no effect on the ACh-mediated vasodilation in the iliac artery, and generic inhibition of NOS with N ω -nitro-l-arginine (3 × 10 -4 M) significantly inhibited the vasodilation, indicating NO signaling. In contrast to the iliac artery, ACh-mediated vasodilation of the sciatic artery had a significant endothelium-dependent component. Interestingly, the vasodilation was not significantly affected by N ω -nitro-l-arginine, but it was significantly inhibited by the specific NOS1 inhibitor N 5 -(1-imino-3-butenyl)-l-ornithine (vinyl-l-NIO, 10 -4 M). ODQ mostly inhibited the ACh-mediated vasodilation. In addition, indomethacin also significantly inhibited the ACh-mediated vasodilation, indicating a role for prostaglandins in the sciatic artery. This study found that the mechanisms of vasodilation in the hindlimb vasculature of R. marina vary with vessel size and that the endothelium is involved in vasodilation in the smaller sciatic artery. |
| فهرسة مساهمة: | Keywords: acetylcholine; amphibian; endothelium; nitric oxide; vasodilation |
| المشرفين على المادة: | 0 (1H-(1,2,3)oxadiazolo(4,4-a)quinoxalin-1-one) 0 (Oxadiazoles) 0 (Quinoxalines) 2149-70-4 (Nitroarginine) EC 1.14.13.39 (Nitric Oxide Synthase) |
| تواريخ الأحداث: | Date Created: 20190627 Date Completed: 20200407 Latest Revision: 20200930 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1152/ajpregu.00319.2018 |
| PMID: | 31242019 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1490 |
|---|---|
| DOI: | 10.1152/ajpregu.00319.2018 |