دورية أكاديمية
A murine femoral ostectomy model with hardware exchange to assess antibiotic-impregnated spacers for implant-associated osteomyelitis.
| العنوان: | A murine femoral ostectomy model with hardware exchange to assess antibiotic-impregnated spacers for implant-associated osteomyelitis. |
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| المؤلفون: | Trombetta RP, de Mesy Bentley KL, Schwarz EM, Kate SL, Awad HA; University of Rochester Medical Centre, 601 Elmwood Avenue, Box 665, Rochester, NY 14642, USA.hani_awad@urmc.rochester.edu. |
| المصدر: | European cells & materials [Eur Cell Mater] 2019 Jun 27; Vol. 37, pp. 431-443. Date of Electronic Publication: 2019 Jun 27. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Forum Multimedia Publishing LLC Country of Publication: Switzerland NLM ID: 100973416 Publication Model: Electronic Cited Medium: Internet ISSN: 1473-2262 (Electronic) Linking ISSN: 14732262 NLM ISO Abbreviation: Eur Cell Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Charlottesville, VA : Forum Multimedia Publishing LLC Original Publication: [Glasgow] Scotland : European Cells & Materials, [2001]- |
| مواضيع طبية MeSH: | Anti-Bacterial Agents/*pharmacology , Femur/*microbiology , Osteomyelitis/*drug therapy , Prostheses and Implants/*microbiology , Prosthesis-Related Infections/*drug therapy, Animals ; Bone Cements/pharmacology ; Disease Models, Animal ; Female ; Mice ; Mice, Inbred BALB C ; Osteomyelitis/microbiology ; Prosthesis-Related Infections/microbiology ; Staphylococcal Infections/drug therapy ; Staphylococcus aureus/drug effects ; Titanium/pharmacology |
| مستخلص: | Implant-associated osteomyelitis is a chronic infection that complicates orthopaedic surgeries. Once infected, 50 % of patients suffer treatment failure, resulting in high healthcare costs. While various small animal models have been developed to investigate the efficacy of prophylactic and therapeutic treatments, the minute scale of murine-model bone and hardware has been prohibitive for evaluating interventions with a complete implant exchange in the setting of an infected critical defect. To address this, the aim of the present study was to develop a murine femur model in which an initial mid-diaphyseal infection was established by surgical implantation of a titanium screw contaminated with bioluminescent Staphylococcus aureus (Xen36). 7 d after the infection was established, an ostectomy was performed to remove the middle segment (3 mm flanking the infected screw hole) and a bone-cement spacer, with or without impregnated gentamicin, was secured with a plate and screws to fix the septic segmental defect. Longitudinal bioluminescent imaging revealed a significant decrease in Xen36 growth following one-stage revision, with the antibiotic-impregnated spacer treated systemically with vancomycin (p < 0.05). This result was corroborated by a significant decrease in colony forming units (CFU) recovered from spacer, bone, soft tissue and hardware 12 d post-operative (p < 0.05). However, ~ 105 CFU/g Xen36 still persisted within the bone despite a clinical therapeutic regimen. Therefore, the model enables the investigation of new therapeutic strategies to improve upon the current standard of care in a mouse model of implant-associated osteomyelitis that employs reconstruction of a critical defect. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Bone Cements) D1JT611TNE (Titanium) |
| تواريخ الأحداث: | Date Created: 20190628 Date Completed: 20200529 Latest Revision: 20200529 |
| رمز التحديث: | 20240628 |
| DOI: | 10.22203/eCM.v037a26 |
| PMID: | 31243755 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1473-2262 |
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| DOI: | 10.22203/eCM.v037a26 |