دورية أكاديمية
Differential expression of H-Y antigen on lymphocyte subsets: analysis by flow cytometry.
| العنوان: | Differential expression of H-Y antigen on lymphocyte subsets: analysis by flow cytometry. |
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| المؤلفون: | Jaswaney VL; Center for Reproductive Biology, Spring Creek Ranch, Collierville, TN 38017., Wachtel SS |
| المصدر: | Differentiation; research in biological diversity [Differentiation] 1987; Vol. 35 (2), pp. 115-21. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 0401650 Publication Model: Print Cited Medium: Print ISSN: 0301-4681 (Print) Linking ISSN: 03014681 NLM ISO Abbreviation: Differentiation Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Elsevier Original Publication: London, Macmillan Journals. |
| مواضيع طبية MeSH: | H-Y Antigen/*analysis , Lymphocytes/*immunology, Adolescent ; Adult ; Antibodies, Monoclonal/isolation & purification ; Female ; Flow Cytometry/methods ; Fluorescein-5-isothiocyanate ; Fluoresceins ; Fluorescent Antibody Technique ; Fluorescent Dyes ; Humans ; Lymphocytes/classification ; Lymphocytes/cytology ; Male ; Middle Aged ; Reference Values ; Sex Factors ; Thiocyanates |
| مستخلص: | Expression of H-Y antigen in human white blood cells was measured using flow cytometry with monoclonal antibodies. In this system, lymphocytes were stained preferentially in the male, and to a lesser extent in the female. Analysis of the lymphocyte subsets with biotinylated H-Y antibody conjugated with streptavidin-fluorescein isothiocyanate (FITC) and subset-specific antibody conjugated with phycoerythrin derivative (RD1) revealed differential expression of H-Y among the subsets of the male. In samples from eight men, 41.1% +/- 21.7% of B cells (B1) were stained, compared with 20.7% +/- 12.8% of cytotoxic-suppressor T cells (T8) and 5.4% +/- 3.0% of helper-inducer T cells (T4). In samples from seven women, 12.4% +/- 10.9% of B cells were stained, but staining of T cells was negligible. |
| معلومات مُعتمدة: | AI-23479 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Fluoresceins) 0 (Fluorescent Dyes) 0 (H-Y Antigen) 0 (Thiocyanates) I223NX31W9 (Fluorescein-5-isothiocyanate) |
| تواريخ الأحداث: | Date Created: 19870101 Date Completed: 19880425 Latest Revision: 20190919 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1111/j.1432-0436.1987.tb00158.x |
| PMID: | 3127261 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0301-4681 |
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| DOI: | 10.1111/j.1432-0436.1987.tb00158.x |