دورية أكاديمية
أعرض في EDS

The molecular architecture of engulfment during Bacillus subtilis sporulation.

التفاصيل البيبلوغرافية
العنوان: The molecular architecture of engulfment during Bacillus subtilis sporulation.
المؤلفون: Khanna K; Division of Biological Sciences, University of California, San Diego, La Jolla, United States., Lopez-Garrido J; Division of Biological Sciences, University of California, San Diego, La Jolla, United States., Zhao Z; Division of Biological Sciences, University of California, San Diego, La Jolla, United States., Watanabe R; Division of Biological Sciences, University of California, San Diego, La Jolla, United States., Yuan Y; Division of Biological Sciences, University of California, San Diego, La Jolla, United States., Sugie J; Division of Biological Sciences, University of California, San Diego, La Jolla, United States., Pogliano K; Division of Biological Sciences, University of California, San Diego, La Jolla, United States., Villa E; Division of Biological Sciences, University of California, San Diego, La Jolla, United States.
المصدر: ELife [Elife] 2019 Jul 08; Vol. 8. Date of Electronic Publication: 2019 Jul 08.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: Bacillus subtilis/*metabolism , Bacterial Proteins/*metabolism , Cell Wall/*metabolism , Peptidoglycan/*metabolism , Spores, Bacterial/*metabolism, Bacillus subtilis/physiology ; Bacillus subtilis/ultrastructure ; Cell Membrane/ultrastructure ; Cell Wall/ultrastructure ; Cryoelectron Microscopy/methods ; Electron Microscope Tomography/methods ; Spores, Bacterial/ultrastructure
مستخلص: The study of bacterial cell biology is limited by difficulties in visualizing cellular structures at high spatial resolution within their native milieu. Here, we visualize Bacillus subtilis sporulation using cryo-electron tomography coupled with cryo-focused ion beam milling, allowing the reconstruction of native-state cellular sections at molecular resolution. During sporulation, an asymmetrically-positioned septum generates a larger mother cell and a smaller forespore. Subsequently, the mother cell engulfs the forespore. We show that the septal peptidoglycan is not completely degraded at the onset of engulfment. Instead, the septum is uniformly and only slightly thinned as it curves towards the mother cell. Then, the mother cell membrane migrates around the forespore in tiny finger-like projections, whose formation requires the mother cell SpoIIDMP protein complex. We propose that a limited number of SpoIIDMP complexes tether to and degrade the peptidoglycan ahead of the engulfing membrane, generating an irregular membrane front.
Competing Interests: KK, JL, ZZ, RW, YY, JS, KP, EV No competing interests declared
(© 2019, Khanna et al.)
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معلومات مُعتمدة: National Institute of Health's Director's New Innovator Award (1DP2GM123494) United States NH NIH HHS; RO1-GM057045 United States NH NIH HHS; R01 GM057045 United States GM NIGMS NIH HHS; DP2 GM123494 United States GM NIGMS NIH HHS; 1DP2GM123494 International National Institute of Health's Director's New Innovator Award
فهرسة مساهمة: Keywords: B. subtilis; Bacillus; cryo-electron tomography; cryo-focused ion beam milling; engulfment; infectious disease; membrane migration; microbiology; molecular biophysics; peptidoglycan; spoIIDMP; structural biology
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Peptidoglycan)
تواريخ الأحداث: Date Created: 20190709 Date Completed: 20200302 Latest Revision: 20220130
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6684271
DOI: 10.7554/eLife.45257
PMID: 31282858
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.45257