دورية أكاديمية
The role of Patronin in Drosophila mitosis.
| العنوان: | The role of Patronin in Drosophila mitosis. |
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| المؤلفون: | Pavlova GA; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia., Razuvaeva AV; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.; Novosibirsk State University, Novosibirsk, 630090, Russia., Popova JV; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia., Andreyeva EN; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia., Yarinich LA; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.; Novosibirsk State University, Novosibirsk, 630090, Russia., Lebedev MO; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.; Novosibirsk State University, Novosibirsk, 630090, Russia., Pellacani C; IBPM CNR and Department of Biology and Biotechnology, Sapienza University of Rome, 00185, Rome, Italy., Bonaccorsi S; IBPM CNR and Department of Biology and Biotechnology, Sapienza University of Rome, 00185, Rome, Italy., Somma MP; IBPM CNR and Department of Biology and Biotechnology, Sapienza University of Rome, 00185, Rome, Italy., Gatti M; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. maurizio.gatti@uniroma1.it.; IBPM CNR and Department of Biology and Biotechnology, Sapienza University of Rome, 00185, Rome, Italy. maurizio.gatti@uniroma1.it., Pindyurin AV; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. a.pindyurin@mcb.nsc.ru.; Novosibirsk State University, Novosibirsk, 630090, Russia. a.pindyurin@mcb.nsc.ru. |
| المصدر: | BMC molecular and cell biology [BMC Mol Cell Biol] 2019 Apr 17; Vol. 20 (Suppl 1), pp. 7. Date of Electronic Publication: 2019 Apr 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101741148 Publication Model: Electronic Cited Medium: Internet ISSN: 2661-8850 (Electronic) Linking ISSN: 26618850 NLM ISO Abbreviation: BMC Mol Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : BioMed Central, [2019]- |
| مواضيع طبية MeSH: | Drosophila Proteins/*metabolism , Drosophila melanogaster/*cytology , Microtubule-Associated Proteins/*metabolism , Mitosis/*physiology, Animals ; Cell Cycle Proteins/metabolism ; Cell Line ; Centrosome/metabolism ; Chromosome Segregation ; Kinesins/metabolism ; Microtubules/metabolism ; Polymerization ; Protein Binding ; Spindle Apparatus/metabolism |
| مستخلص: | Background: The calmodulin-regulated spectrin-associated proteins (CAMSAPs) belong to a conserved protein family, which includes members that bind the polymerizing mcrotubule (MT) minus ends and remain associated with the MT lattice formed by minus end polymerization. Only one of the three mammalian CAMSAPs, CAMSAP1, localizes to the mitotic spindle but its function is unclear. In Drosophila, there is only one CAMSAP, named Patronin. Previous work has shown that Patronin stabilizes the minus ends of non-mitotic MTs and is required for proper spindle elongation. However, the precise role of Patronin in mitotic spindle assembly is poorly understood. Results: Here we have explored the role of Patronin in Drosophila mitosis using S2 tissue culture cells as a model system. We show that Patronin associates with different types of MT bundles within the Drosophila mitotic spindle, and that it is required for their stability. Imaging of living cells expressing Patronin-GFP showed that Patronin displays a dynamic behavior. In prometaphase cells, Patronin accumulates on short segments of MT bundles located near the chromosomes. These Patronin "seeds" extend towards the cell poles and stop growing just before reaching the poles. Our data also suggest that Patronin localization is largely independent of proteins acting at the MT minus ends such as Asp and Klp10A. Conclusion: Our results suggest a working hypothesis about the mitotic role of Patronin. We propose that Patronin binds the minus ends within MT bundles, including those generated from the walls of preexisting MTs via the augmin-mediated pathway. This would help maintaining MT association within the mitotic bundles, thereby stabilizing the spindle structure. Our data also raise the intriguing possibility that the minus ends of bundled MTs can undergo a limited polymerization. |
| التعليقات: | Erratum in: BMC Mol Cell Biol. 2019 Jul 8;20(1):24. (PMID: 31286886) |
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| فهرسة مساهمة: | Keywords: Asp; Augmin; CAMSAP proteins; Dgt6; Drosophila; Klp10A; Microtubules; Mitosis; Patronin; S2 cells |
| المشرفين على المادة: | 0 (Cell Cycle Proteins) 0 (Dgt6 protein, Drosophila) 0 (Drosophila Proteins) 0 (Microtubule-Associated Proteins) 0 (asp protein, Drosophila) 0 (mi protein, Drosophila) 0 (patronin protein, Drosophila) EC 3.6.1.- (KLP10A protein, Drosophila) EC 3.6.4.4 (Kinesins) |
| تواريخ الأحداث: | Date Created: 20190710 Date Completed: 20191211 Latest Revision: 20211204 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC6469034 |
| DOI: | 10.1186/s12860-019-0189-0 |
| PMID: | 31284878 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2661-8850 |
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| DOI: | 10.1186/s12860-019-0189-0 |