دورية أكاديمية
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
| العنوان: | Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
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| المؤلفون: | Macedo TJS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Santos JMS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Bezerra MÉS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Menezes VG; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Gouveia BB; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Barbosa LMR; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Lins TLBG; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Monte APO; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Barberino RS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Batista AM; Laboratory of Animal Reproduction, Federal Rural University of Pernambuco, Recife, PE, Brazil., Barros VRP; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Wischral A; Laboratory of Animal Reproduction, Federal Rural University of Pernambuco, Recife, PE, Brazil., Queiroz MAA; Laboratory of Bromatology and Animal Nutrition, Federal University of São Francisco Valley, Petrolina, PE, Brazil., Araújo GGL; Embrapa Semi-Arid, Petrolina, PE, Brazil., Matos MHT; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil. Electronic address: helena.matos@univasf.edu.br. |
| المصدر: | Molecular and cellular endocrinology [Mol Cell Endocrinol] 2019 Sep 15; Vol. 495, pp. 110506. Date of Electronic Publication: 2019 Jul 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: North Holland Publishing Country of Publication: Ireland NLM ID: 7500844 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8057 (Electronic) Linking ISSN: 03037207 NLM ISO Abbreviation: Mol Cell Endocrinol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : North Holland Publishing Original Publication: Amsterdam, North-Holland. |
| مواضيع طبية MeSH: | Cell Differentiation*, Leptin/*metabolism , Leptin/*pharmacology , Oocytes/*cytology , Oocytes/*metabolism , Ovarian Follicle/*growth & development , Receptors, Leptin/*metabolism , Sheep/*metabolism, Animals ; Chromatin/metabolism ; Female ; Glutathione/metabolism ; In Vitro Oocyte Maturation Techniques ; Intracellular Space/metabolism ; Meiosis ; Mitochondria/metabolism ; Ovarian Follicle/cytology |
| مستخلص: | The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM + alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM + . 25 ng/mL leptin showed a higher percentage of MII than the α-MEM + . In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Follicular development; Immunohistochemistry; Oocyte; Ovary; Sheep |
| المشرفين على المادة: | 0 (Chromatin) 0 (Leptin) 0 (Receptors, Leptin) GAN16C9B8O (Glutathione) |
| تواريخ الأحداث: | Date Created: 20190712 Date Completed: 20200601 Latest Revision: 20200601 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.mce.2019.110506 |
| PMID: | 31295510 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-8057 |
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| DOI: | 10.1016/j.mce.2019.110506 |